组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2017, Vol. 13 ›› Issue (2): 66-69.doi: 10.3969/j.issn.1673-0364.2017.02.002

• 论著 • 上一篇    下一篇

大孔GT/PCL 电纺材料作为软骨组织工程支架的可行性研究

郑蕊，赵仕芳，朱月倩，周广东   

  1. 上海交通大学医学院附属第九人民医院皮肤科；上海市组织工程研究重点实验室；组织工程国家工程中心；东华大学化学化工与生物工程学院
  • 发布日期:2020-07-23

The Feasibility of the Macroporous GT/PCL Electrospun Material as Scaffold for Cartilage Tissue Engineering

ZEHNG Rui1,3,4, ZHAO Shifang2, ZHU Yueqian3,4, ZHOU Guangdong3,4   

  1. 1 Department of Dermatology, Shanghai Ninth People＇s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China; 2 College of Chemistry, Chemical Engineering ＆ Biotechnology,Donghua University, Shanghai 201620, China; 3 Shanghai Key Laboratory of Tissue Engineering, Shanghai 200011, China; 4 National Tissue Engineering Center of China, Shanghai 200241, China.）
  • Published:2020-07-23
  • Contact: 国家自然科学基金；江苏省自然科学基金

摘要： 目的 探讨大孔径GT/PCL电纺材料作为软骨组织工程支架的可行性。方法 取猪耳郭软骨细胞为种子细胞。大孔径GT/PCL电纺纳米材料设为实验组,无纺布GT/PCL电纺纳米材料设为对照组,对二者的形貌特征和孔隙率进行观察比较,在二维平面比较二者与软骨细胞的黏附率和细胞增殖能力,并在三维空间比较软骨细胞的浸润情况。结果扫描电镜显示,实验组材料孔径明显大于对照组,孔隙率测试数据也支持该结果;二维平面上,两组材料对软骨细胞的黏附和增殖能力无显著性差异,SEM显示实验组软骨细胞可长入材料内部;三维空间中,软骨细胞在实验组的浸润深度明显大于对照组。结论 大孔GT/PCL电纺材料不影响软骨细胞的黏附与增殖,明显利于软骨细胞的长入和浸润,在软骨组织工程的应用上具有良好的前景。

关键词: 大孔电纺材料, 软骨组织工程, 孔隙率

Abstract: Objective To investigate the feasibility of the macroporous gelatin/polycaprolactone (GT/PCL) electrospun material as scaffold for cartilage tissue engineering. Methods The auricular chondrocytes of porcine were harvested as seed cells. Two kinds of electrospun membrane were prepared and compared: the macroporous membranes (experimental group) and the non-woven membranes (control group) as scaffolds. The structure and porosity of the two materials were observed. Adhesion and proliferation of chondrocytes on the two membranes were examined in two-dimensional level (2D). And the depth of cellular infiltration were observed in three-dimensional level (3D). Results Scanning electron microscope (SEM) results suggested that the pore size of the experimental group was significantly bigger than the control group, and the data of porosity supported this result. In 2D level, there was no significant difference in adhesion and proliferation of chondrocytes between the two groups. However, SEM showed that chondrocytes grew into the interior of the macroporous material. In 3D level, the infiltration depth of chondrocytes in the experimental group was significantly greater than that in the control group. Conclusion The macroporous GT/PCL membranes do not affect the adhesion and proliferation of chondrocytes, and it is beneficial to the growth and infiltration of chondrocytes. It could be a promising scaffold for cartilage tissue engineering.

Key words: Macroporous electrospun material, Tissue engineering, Porosity

中图分类号: