组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2014, Vol. 10 ›› Issue (2): 73-76.doi: 10.3969/j.issn.1673-0364.2014.02.003

• 论著 • 上一篇    下一篇

应用胚胎皮肤细胞修复小鼠皮肤缺损的实验研究

郭相凯，韩志华，段惠川，钱德检，郗林鹤，孟飞，刘炬，王彦   

  1. 山东省医学科学院；济南大学,山东省医学科学院医学与生命科学学院；上海交通大学医学院附属第九人民医院；山东省千佛山医院创伤急诊外科；山东省千佛山医院整形外科；山东省千佛山医院医学研究中心
  • 发布日期:2020-07-23

The Application of Embryonic Skin Cell in Repairing Skin Defect of Mouse

GUO Xiangkai,HAN Zhihua,DUAN Huichuan,QIAN Dejian,XI Linhe,MENG Fei,LIU Ju,WANG Yan   

  1. Shangdong Academy of Medical Sciences;School of Medicine and Life Sciences,University of Jinan-Shangdong Academy of Medical Sciences; Department of Plastic Surgery,Qianfoshan Hospital Affiliated to Shandong University;Shanghai Jiaotong University School of Medicine,Shanghai Ninth People’s Hospital; Department of Trauma and Emergency Surgery,Qianfoshan Hospital Affiliated to Shandong University;Medical Research Center,Qianfoshan Hospital Affiliated to Shandong University;
  • Published:2020-07-23
  • Contact: 山东省科技发展计划项目(2011GSF11839)；山东省自然科学基金(ZR2009CQ001)

摘要： 目的探索胚胎皮肤细胞修复小鼠背部皮肤缺损的疗效。方法建立小鼠皮肤损伤模型,将小鼠胚胎皮肤的细胞植入小鼠皮肤损伤处(Nor-T组,n=6),与不作处理的小鼠(Nor组,n=6)进行皮肤损伤愈合的比较。分别在术后第1、2、3周采集标本,比较Nor-T组与Nor组创面愈合的情况,以免疫组织化学方法检测各组愈合过程中,TGF-β1和TGF-β3因子的表达情况。结果术后第1周,Nor-T组在创伤处形成再生皮肤覆盖创面;Nor组创面由痂皮覆盖;术后第2周,Nor-T组再生皮肤完全形成;Nor组瘢痕愈合。术后第3周,Nor-T组再生皮肤上生长出黑色毛发;而Nor组没有毛发生长。Nor-T组中,TGF-β1在术后第1周表达阳性,TGF-β3在术后第2周表达阳性。结论胚胎皮肤细胞具有形成再生皮肤和修复皮肤缺损的能力。

关键词: 胚胎皮肤细胞, 再生皮肤, 瘢痕形成

Abstract: Objective To explore the efficacy of repairing skin defect of mouse by using embryonic skin cell. Methods Animal model of back skin damage in mice was built. The embryonic skin cells were transplanted into the back skin damage of mice as treatment group (Nor-T group, n=6) and the mice in control group (Nor group, n=6) were received no treatment. The wound healing situation were observed 1, 2 and 3 weeks after operation. The expression of TGF-βwas detected 1 and 2 weeks after operation. Results The regenerative skin growth was observed on the wound area in Nor-T group, while only crusta was observed on the wound area in Nor group 1 week after operation. The regenerative skin merged with surrounding skin in Nor-T group, while the wound healed with scar in in Nor group 2 weeks after operation. The hair was observed in Nor-T group, while no hair was observed in Nor group 3 weeks after operation. In Nor-T group, TGF-β1 was expressed 1 week after operation and TGF- β3 was expressed 2 week after operation. Conclusion The embryonic skin cells possess the ability of regenerative skin formation, and TGF-βhas different expression at different wound healing phase.

Key words: Embryonic skin cell, Regenerative skin, Scar formation

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