组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2010, Vol. 6 ›› Issue (6): 323-326.doi: 10.3969/j.issn.1673-0364.2010.06.006

• 论著 • 上一篇    下一篇

应用噬菌体随机12肽库筛选TβR Ⅱ特异性序列

宗宪磊，姜笃银，蔡景龙，陈莹   

  1. 中国医学科学院北京协和医学院整形外科医院；山东大学组织工程研究所
  • 发布日期:2020-07-23

Isolation of Specific Sequence of TGF-β Receptor Ⅱ Using a Phage 12-mer Display Peptide Library

ZONG Xianlei1,JIANG Duyin2,CAI Jinglong1,CHEN Ying1   

  1. 1 Plastic Surgery Hospital,Chinese Academy Medical Sciences,Beijing 100144,China;2 Institute of Tissue Engineering of Shandong University,Jinan 250033,China
  • Published:2020-07-23
  • Contact: 国家自然科学基金资助项目(30772258,81071560)

摘要： 目的从噬菌体随机12肽库中筛选TGF-β1Ⅱ型受体(TGF-beta receptorⅡ,TβRⅡ)的特异性序列,评估其对瘢痕疙瘩成纤维细胞的作用。方法以人TGF-β1单克隆抗体为靶,在噬菌体随机12肽库中进行4轮生物筛选;应用ELISA法挑选结合活性较好的单克隆噬菌体,进行DNA序列分析;MTT法评估TGF-β1单克隆噬菌体对瘢痕疙瘩成纤维细胞的生物学效应。结果测序共获得5种类似于TβRⅡ的特异性序列。MTT结果显示其中有3种噬菌体模拟肽能够抑制瘢痕疙瘩成纤维细胞增殖。结论具有TβRⅡ特异性序列的噬菌体模拟肽能够抑制瘢痕疙瘩成纤维细胞增殖。

关键词: 噬菌体随机12肽库, 转化生长因子-βⅡ型受体, 瘢痕疙瘩成纤维细胞

Abstract: Objective To isolate the specific sequence of TGF-β receptor Ⅱ(TβRⅡ) from a phage 12-mer display peptide library and to evaluate their effects on keloid fibroblasts.Methods A phage 12-mer display peptide library was screened for four rounds using monoclonal anti-human TGF-β1 as the target.ELISA was performed to select monoclonal phages with good association for DNA sequencing.MTT assay was used to detect the effects of monoclonal phages on keloid fibroblasts.Results Five specific sequences were obtained,which were similar to TβRⅡ.The results of MTT assay showed that three of the five phage model peptides could inhibit proliferation of keloid fibroblasts.Conclusion Phage model peptides with specific sequences of TβRⅡ can inhibit proliferation of keloid fibroblasts.

Key words: Phage 12-mer display peptide library, TGF-β receptor Ⅱ, Keloid fibroblast

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