综述

modRNA技术:一种蛋白表达新方法

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  • 上海交通大学医学院附属上海儿童医学中心转化医学研究所;上海交通大学医学院附属上海儿童医学中心胸心外科;上海市组织工程研究重点实验室
付炜(E-mail:fuweizhulu@163.com)。

收稿日期: 2019-02-03

  修回日期: 2019-03-16

  网络出版日期: 2019-04-20

modRNA Technology:A Newly-Developed Method of Protein Expression

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  • Institute of Pediatric Translational Medicine; Department of Pediatric Cardiothoracic Surgery,Shanghai Children's Medical Center,Shanghai Jiaotong University School of Medicine,Shanghai 200127,China;Shanghai Key Laboratory of Tissue Engineering,Shanghai 200011,China)

Received date: 2019-02-03

  Revised date: 2019-03-16

  Online published: 2019-04-20

摘要

早在上世纪90年代,注射裸信使核糖核酸(mRNA)已经能够在体内实现外源性蛋白的表达。然而,mRNA的不稳定性及高度免疫原性阻碍了这项技术的进一步发展与应用。近年来,随着对mRNA结构的认识深入及核酸技术的进步,可化学合成并修饰信使核糖核酸,即Chemically synthetic modified message RNA(modRNA),modRNA兼具稳定性、低免疫原性及良好的蛋白翻译表的优势。我们对其研究现状、存在问题进行综述,并对可能的改进方向进行展望。

本文引用格式

颜冰倩,综述,付炜,审校 . modRNA技术:一种蛋白表达新方法[J]. 组织工程与重建外科杂志, 2019 , 15(2) : 107 -110 . DOI: 10.3969/j.issn.1673-0364.2019.02.012

Abstract

Earlier in the 1990s,direct injection of naked mRNA has been proved to be an approach to achieve the exogenous gene expression in vivo.However,the instability and high-immunogenicity of mRNA becomes the main challenges of further development and application of this technology.Recently,with the in-depth research of mRNA structure and the advancement of nucleic acid technologies,chemically synthetic modified message RNA(modRNA)has emerged as a newly-developed gene vector,featuring stability,low-immunogenicity and high expression of target proteins.Based on the related researches,the concept,structure,mechanism and research status of modRNA were concluded,and the advantages and flaws along with the potential improvement direction were put forward in this paper.
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