组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2019, Vol. 15 ›› Issue (2): 107-110.doi: 10.3969/j.issn.1673-0364.2019.02.012

• 综述 • 上一篇    下一篇

modRNA技术：一种蛋白表达新方法

颜冰倩，综述，付炜，审校   

  1. 上海交通大学医学院附属上海儿童医学中心转化医学研究所；上海交通大学医学院附属上海儿童医学中心胸心外科；上海市组织工程研究重点实验室
  • 收稿日期:2019-02-03 修回日期:2019-03-16 出版日期:2019-04-20 发布日期:2019-04-20
  • 作者简介:付炜(E-mail:fuweizhulu@163.com)。

modRNA Technology:A Newly-Developed Method of Protein Expression

YAN Bingqian,FU Wei   

  1. Institute of Pediatric Translational Medicine; Department of Pediatric Cardiothoracic Surgery,Shanghai Children's Medical Center,Shanghai Jiaotong University School of Medicine,Shanghai 200127,China;Shanghai Key Laboratory of Tissue Engineering,Shanghai 200011,China)
  • Received:2019-02-03 Revised:2019-03-16 Online:2019-04-20 Published:2019-04-20
  • Contact: 上海市浦江人才计划(18PJD031)；上海交通大学医工交叉项目(YG2016MS28)；上海交通大学医学院转化医学协同创新中心合作研究项目(TM201821)；上海交通大学医学院国际和地区联合研究项目；上海交通大学医学院紧缺专业硕士研究生临床研究能力提升计划(EKKY2018002DGD)

摘要： 早在上世纪90年代,注射裸信使核糖核酸(mRNA)已经能够在体内实现外源性蛋白的表达。然而,mRNA的不稳定性及高度免疫原性阻碍了这项技术的进一步发展与应用。近年来,随着对mRNA结构的认识深入及核酸技术的进步,可化学合成并修饰信使核糖核酸,即Chemically synthetic modified message RNA(modRNA),modRNA兼具稳定性、低免疫原性及良好的蛋白翻译表的优势。我们对其研究现状、存在问题进行综述,并对可能的改进方向进行展望。

关键词: 化学合成修饰信使核糖核酸, 基因表达, 机制

Abstract: Earlier in the 1990s,direct injection of naked mRNA has been proved to be an approach to achieve the exogenous gene expression in vivo.However,the instability and high-immunogenicity of mRNA becomes the main challenges of further development and application of this technology.Recently,with the in-depth research of mRNA structure and the advancement of nucleic acid technologies,chemically synthetic modified message RNA(modRNA)has emerged as a newly-developed gene vector,featuring stability,low-immunogenicity and high expression of target proteins.Based on the related researches,the concept,structure,mechanism and research status of modRNA were concluded,and the advantages and flaws along with the potential improvement direction were put forward in this paper.

Key words: Chemically synthetic modified message RNA, Gene expression, Mechanism

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