目的探讨以改进的软骨细胞膜片技术为基础,以大型哺乳动物耳软骨细胞为种子细胞,体外构建与羊气管实际管径相当的管状软骨的可行性。方法山羊耳郭软骨细胞体外培养1~2周后,软骨细胞膜片体外塑形,体外培养6周时收取标本,观察大体外观及弹性,检查软骨形成情况。结果体外培养6周时卷起的膜片已融合为完整的白色半透明管状组织,具有一定的生物力学强度;组织学检测证实,体外构建的管状组织的管壁为连续的软骨样组织。结论改进后的软骨细胞膜片技术具有原代细胞用量少、体外培养时间短、炎症反应少等优点,有更广泛的临床应用前景。

Objective To explore the feasibility of constructing tubular cartilage with a diameter similar to goat tracheal in vitro by large mammalian auricular cartilage,based on improved cell-sheet technology.Methods The chondrocyte cell-sheet was cultured for 1-2 weeks in vitro,and was shaped as tubular cartilage tissue.Samples were collected at 6 weeks.The general appearance and elasticity were observed,and the formation of cartilage was examined.Results After cultured for 6 weeks in vitro,the cell-sheet had intergraded to a white translucent tubular tissue.The tubular tissue had certain biomechanical strength similar to mature cartilage tissues,and the histology analysis displayed continuous cartilage phenotype.Conclusion The improved cartilage cell-sheet technology has a series of advantage including limited defect in donor site,short period in vitro culture,less inflammatory response in vivo and so on.This technology would provide a promising approach for the regeneration of cartilage in vivo.