组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2018, Vol. 14 ›› Issue (3): 123-126.doi: 10.3969/j.issn.1673-0364.2018.03.002

• 论著 • 上一篇    下一篇

利用软骨细胞膜片技术体外构建管状软骨组织的实验研究

刘浥，李丹，殷宗琦，周广东，曹谊林   

  1. 中国医学科学院皮肤病研究所整形外科；上海交通大学医学院附属第九人民医院整复外科上海市组织工程重点实验室；组织工程国家工程中心
  • 收稿日期:2018-03-22 发布日期:2020-07-23
  • 作者简介:周广东（E-mail：guangdongzhou@126．com）;曹谊林（E-mail：yilincao@yahoo．com）。

A Study of Constructing Tubular Tissue Engineered Cartilage by Using Chondrocytes Cell-Sheet Approach

LIU Yi,LI Dan,YIN Zongqi,ZHOU Guangdong,CAO Yilin   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College; Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering;National Tissue Engineering Center of China
  • Received:2018-03-22 Published:2020-07-23
  • Contact: 国家自然科学基金(31400830，81270070，81371703，81371701)；协和青年基金项目(3332014047)；上海市产学研医项目(15DZ1941600)

摘要： 目的探讨以改进的软骨细胞膜片技术为基础,以大型哺乳动物耳软骨细胞为种子细胞,体外构建与羊气管实际管径相当的管状软骨的可行性。方法山羊耳郭软骨细胞体外培养1~2周后,软骨细胞膜片体外塑形,体外培养6周时收取标本,观察大体外观及弹性,检查软骨形成情况。结果体外培养6周时卷起的膜片已融合为完整的白色半透明管状组织,具有一定的生物力学强度;组织学检测证实,体外构建的管状组织的管壁为连续的软骨样组织。结论改进后的软骨细胞膜片技术具有原代细胞用量少、体外培养时间短、炎症反应少等优点,有更广泛的临床应用前景。

关键词: 组织工程软骨, 细胞膜片技术, 管状软骨

Abstract: Objective To explore the feasibility of constructing tubular cartilage with a diameter similar to goat tracheal in vitro by large mammalian auricular cartilage,based on improved cell-sheet technology.Methods The chondrocyte cell-sheet was cultured for 1-2 weeks in vitro,and was shaped as tubular cartilage tissue.Samples were collected at 6 weeks.The general appearance and elasticity were observed,and the formation of cartilage was examined.Results After cultured for 6 weeks in vitro,the cell-sheet had intergraded to a white translucent tubular tissue.The tubular tissue had certain biomechanical strength similar to mature cartilage tissues,and the histology analysis displayed continuous cartilage phenotype.Conclusion The improved cartilage cell-sheet technology has a series of advantage including limited defect in donor site,short period in vitro culture,less inflammatory response in vivo and so on.This technology would provide a promising approach for the regeneration of cartilage in vivo.

Key words: Tissue engineered cartilage, Cell-sheet technology, Tubular cartilage

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