目的 设计并构筑具有模拟尿道组织微环境的三维微/纳支架材料。方法 通过对尿道脱细胞基质多尺度结构分析,设计仿尿道组织的双层细菌纤维素(Baterial cellulose,BC)基微/纳组织工程尿道支架。采用模板合成法,以明胶多孔支架为模板,在其上静态培养制备兼具微米多孔与纳米纤维的双层BC基微/纳支架材料。结果 双层BC基微/纳支架材料的致密层为细菌纤维素膜,疏松层为相互连通的多孔结构,平均孔径为(167±56)μm,同时在其微米级孔壁表面修饰着模拟天然细胞外基质(Extracellular matrix,ECM)结构的纳米纤维网络,纳米纤维直径为(20~40)nm。结论 采用模板合成法,成功构建出了兼具宏观双层结构、微米级多孔和纳米级纤维的双层BC微/纳组织工程尿道支架。

Objective To design and fabrication of the 3D scaffolds that exhibit biomimetic multi-scale structures composed ofmicroporous structure with nanofibers for urethral tissue.Methods By analyzing themulti-scale structures of urethral acellularmatrix,a bilayer scaffold composed ofmicroporous section and nanofibrous dense section to biomimetic urethral tissue was designed.Template biosynthesis was used to fabricate 3D microporous nanofibrous gelatin/BC bilayer scaffolds(BC-Gel/BC)by stationary cultivation usingmicroporous gelatin scaffold as a template.Results The BC-Gel/BC bilayer scaffold composed of dense section of BC nanofibrils network and microporous section of Gel/BC.Themicroporous section with highly interconnected micropore(167±56)μm and surface decorated on themicropore wall by BC nanofibers (20-40)nm were fabricated,which were remarkably similar structure to the native extracellularmatrix(ECM).Conclusion Using the template biosynthesis method,the BC-Gel/BC bilayer scaffold for urethral tissue engineering can be successfully constructed,which has the bilayer structure,m icro porous and nanofibrous network to biom imetic urethral tissue.