组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2016, Vol. 12 ›› Issue (5): 276-280.doi: 10.3969/j.issn.1673-0364.a448

• 论著 • 上一篇    下一篇

双层细菌纤维素基组织工程尿道支架的设计与初步构建

李喆，吕向国，王宝秀，冯超，陈仕艳，颜志勇，王华平   

  1. 东华大学材料学与工程学院纤维材料改性国家重点实验室；嘉兴学院材料与纺织工程学院；上海交通大学附属第六人民医院泌尿外科
  • 发布日期:2020-07-23

Design and Fabrication of Abacterial Cellulose Bilayer Scaffold for Urethral Tissue Engineering

LI Zhe1,2,LV Xiangguo3,WANG Baoxiu1,FENG Chao3,CHEN Shiyan1,YAN Zhiyong2,WANG Huaping1.   

  1. 1 State Key Laboratory for Modification of Chem ical Fibers and Polymer Materials,College of Materials Science and Engineering,Donghua University, Shanghai 201620,China;2 Materials and Textile Engineering College,Jiaxing University,Jiaxing 314001,China;3 Department of Urology,Affiliated Sixth Peop le's Hospital,Shanghai Jiaotong University,Shanghai 201620,China. Corresponding author: WANG Huaping E-mail:wanghp@dhu.edu.cn).
  • Published:2020-07-23
  • Contact: 博士创新基金（CUSF-DH-D-2015029）；国家自然科学基金（51573024）。

摘要： 目的 设计并构筑具有模拟尿道组织微环境的三维微/纳支架材料。方法 通过对尿道脱细胞基质多尺度结构分析,设计仿尿道组织的双层细菌纤维素(Baterial cellulose,BC)基微/纳组织工程尿道支架。采用模板合成法,以明胶多孔支架为模板,在其上静态培养制备兼具微米多孔与纳米纤维的双层BC基微/纳支架材料。结果 双层BC基微/纳支架材料的致密层为细菌纤维素膜,疏松层为相互连通的多孔结构,平均孔径为(167±56)μm,同时在其微米级孔壁表面修饰着模拟天然细胞外基质(Extracellular matrix,ECM)结构的纳米纤维网络,纳米纤维直径为(20~40)nm。结论 采用模板合成法,成功构建出了兼具宏观双层结构、微米级多孔和纳米级纤维的双层BC微/纳组织工程尿道支架。

关键词: 尿道组织工程, 脱细胞基质, 细菌纤维素, 多尺度结构

Abstract: Objective To design and fabrication of the 3D scaffolds that exhibit biomimetic multi-scale structures composed ofmicroporous structure with nanofibers for urethral tissue.Methods By analyzing themulti-scale structures of urethral acellularmatrix,a bilayer scaffold composed ofmicroporous section and nanofibrous dense section to biomimetic urethral tissue was designed.Template biosynthesis was used to fabricate 3D microporous nanofibrous gelatin/BC bilayer scaffolds(BC-Gel/BC)by stationary cultivation usingmicroporous gelatin scaffold as a template.Results The BC-Gel/BC bilayer scaffold composed of dense section of BC nanofibrils network and microporous section of Gel/BC.Themicroporous section with highly interconnected micropore(167±56)μm and surface decorated on themicropore wall by BC nanofibers (20-40)nm were fabricated,which were remarkably similar structure to the native extracellularmatrix(ECM).Conclusion Using the template biosynthesis method,the BC-Gel/BC bilayer scaffold for urethral tissue engineering can be successfully constructed,which has the bilayer structure,m icro porous and nanofibrous network to biom imetic urethral tissue.

Key words: Urethral tissue engineering, Acellularmatrix, Bacterial cellulose, Multi-scale structure

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