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小口径聚乳酸-己内酯/纤维蛋白原管形支架的构建及生物相容性评价

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  • 安徽医科大学附属省立医院血管外科;复旦大学附属中山医院血管外科

网络出版日期: 2020-07-23

Establish ment and Biocompatibility of a Small Diameter Tubular Scaffold of Electrospun Poly (L-lactide-co-ε-caprolactone)/fibrinogen Blended Fibers

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  • Department of Vascular Surgery, Provincial Hospital Affiliated to Anhui Medical University; Department of Vascular Surgery, Zhongshan Hospital, Fudan University;

Online published: 2020-07-23

摘要

目的探索静电纺丝技术制备小口径聚乳酸-己内酯[P(LLA-CL)]/纤维蛋白原管形支架的方法,评价支架的生物相容性,探讨其作为血管组织工程材料的可行性。方法以P(LLA-CL)、纤维蛋白原为原料,制备小口径复合管形支架,观察支架的大体形态,并用扫描电镜观察三维结构;利用溶血试验、细胞毒性试验、皮下植入试验,评价支架材料的生物相容性。结果管形支架表面呈网格状三维结构,并有大小不等、互相交通的孔隙,孔径平均直径为(4.56±1.23)μm,表面纤维平均直径(318±56)nm;P(LLA-CL)/纤维蛋白原浸提液溶血率为2.87%±0.49%;细胞毒性实验示P(LLA-CL)/纤维蛋白原浸提液较阴性对照组无明显差异(P>0.05);皮下植入试验显示P(LLA-CL)/纤维蛋白原支架炎症反应轻微,材料逐渐降解。结论通过静电纺丝技术可以构建小口径P(LLA-CL)/纤维蛋白原管形支架,并具有良好的生物相容性,可作为组织工程血管的支架材料。

本文引用格式

方征东,胡何节,董智慧,王晓天,孙小杰,葛新宝 . 小口径聚乳酸-己内酯/纤维蛋白原管形支架的构建及生物相容性评价[J]. 组织工程与重建外科杂志, 2014 , 10(4) : 207 -210 . DOI: 10.3969/j.issn.1673-0364.2014.04.010

Abstract

Objective To fabricate a small diameter tubular scaffold of P (LLA-CL)/fibrinogen by electrospinning fiber technique, to evaluate the biocompatibility of the P (LLA-CL)/fibrinogen scaffold and its feasibility for vascular tissue engineering. Methods A small diameter tubular scaffold was fabricated by co-electrospinning blend of P (LLA-CL) and Fibrinogen. Gross morphology of the scaffold was observed and the 3-dimensional structure was observed by scanning electron microscope (SEM). Biocompatibilities of the tubular scaffold were evaluated in vivo and in vitro by acute hemolysis test, cytotoxicity test, and short-term test of subcutanous implantation. Results A randomly oriented nanofibrous structure with a well interconnected network of pores was observed. The diameter of the fiber at the outer surface was 318 ±56 nm and the average pore diameter was 4.56 ±1.23 μm; Hemolysis rate was 2.87%±0.49%; There was no significant difference of cytotoxicity between the P (LLA-CL)/fibrinogen tubular scaffold and negative control group (P>0.05). After the placement in rat subcutaneous pouches, the scaffolds were gradually biodegraded with little inflammatory reaction. Conclusion The P (LLA-CL)/fibrinogen tubular scaffold can be fabricated by electrospinning fiber technique. It has good biocompatibility and could be potentially used as vascular tissue engineering scaffold material.
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