Abstract:

Objective　Using in-situ bioprinting to repair full-thickness skin wound on the back of nude mice to achieve

in-situ repairing and hair follicle reconstruction. Methods　Dorsal full-thickness skin defects (7 mm in diameter) was created

and covered with CA/BC in nude mice. About 5×105 primary cells from neonatal C57 mice skin were used as seed cells and

transplanted to the wound: 10 points of high-concentration cell plotting was printed in the CA/BC via in-situ bioprinting in ex

perimental group while 1 mL injector was used manually in control group. After 4 weeks, wound repairing and hair growth were

observed, and the structure of new hair follicles was examined by H&E staining. Results　The skin defect wound of the exper

imental group recovered well with vigorous and uniform hair growth, while the hair of the control group was sparse, uneven or

even no hair growth. HE staining showed that there was complete hair follicle structure in the new skin of the experimental group,

while there was almost no hair follicle formation in the control group. Conclusion　The feasibility of in-situ bioprinting tech

nology in skin defect repair and hair follicle reconstruction is preliminarily verified.

Key words:

　Bioprinting, 　In-situ bioprinting, 　Full-thickness skin defect, 　Hair follicle reconstitution