Abstract: Objective　 To explore the in vitro biocompatibility of five different contents of polycaprolactone/reduced graphene oxide （PCL/RGO） nerve duct basement membrane materials with PC12 cells. Methods　Five kinds of PCL/RGO nerve duct basement membrane materials with different RGO contents were prepared by electrospinning technique and cocultured with PC12 cells. The proliferation of PC12 cells was determined by CCK-8 method， the adhesion and reproduction of PC12 cells were observed by scanning electron microscopy， and the cell activity was observed by Live/Dead fluorescence staining. Results　The cell proliferation rate of the five groups was different， but the toxicity grade was 0（ no cytotoxicity）， and the cell proliferation of 0.75%RGO/PCL group was the best. After 5 groups of materials were cultured for 7 days， the cell adhesion growth was significantly increased under electron microscope compared with 1 day and 3 days， and the surface of the materials was fully coated. The Live/Dead fluorescence staining results showed that the survival rate of cells in all groups was above 90%. Conclusion　Five kinds of PCL/RGO composites with different RGO contents all have good biocompatibility， and the composite materials with 0.75%RGO/PCL ratio have a relatively large advantage in promoting cell proliferation and adhesion.

Key words: 　Electrospinning, 　Polycaprolactone, 　Reduced graphene oxide, 　PC12 cells, 　Biocompatibility