Abstract: Fluorescence-guided surgery（ FGS） uses fluorescent contrast agents to visualize the target tissue and provide real-time dynamic images. Its temporal resolution， spatial resolution and application mode are superior to magnetic resonance imaging， computed tomography and ultrasound， and it is mainly used in tumor resection. Currently in reconstructive surgery， surgeons are often involved in the localization and discrimination of blood vessels， lymph vessels， and nerves. In this paper， the application status of FGS in flap surgery， lymphatic and nerve reconstruction from the above three aspects， and the development progress of related fluorescent contrast agents and imaging system were all reviewed.

Key words: Fluorescence-guided surgery, 　Contrast media, 　Flap, 　Lymphedema, 　Peripheral nerve injuries