Journal of Tissue Engineering and Reconstructive Surgery ›› 2024, Vol. 20 ›› Issue (6): 665-.

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Study on lncRNA H19 promoting osteogenic differentiation of placenta-derived mesenchymal stem cells

  

  • Online:2024-12-02 Published:2025-01-03

Abstract:

Objective To observe the effects of lncRNA H19 transfection on the expression of multiple osteogenic and angiogenic factors in human placenta-derived mesenchymal stem cells (P-MSCs), and to provide a preliminary research foundation for the synthesis of bone defect fillers with good osteogenic properties in the future. Methods First, miRNAs that regulate a large number of osteogenic and angiogenic factors were screened, and miR-29b-3p, miR-29c-3p, miR-137-3p, and miR-383-5p were identified as the target miRNAs. Further bioinformatics analysis revealed that lncRNA H19 can
regulate all of these miRNAs. Subsequently, miR-137-3p and miR-383-5p, which regulate a greater number of osteogenic and angiogenic factors, were selected as the targets of H19. Dual luciferase reporter gene assay was used to verify the  regulatory relationship between H19 and miR-383-5p/miR-137-3p. H19 was cloned into lentivirus and transfected into placenta-derived mesenchymal stem cells (PMSCs). The expression levels of miR-137-3p, miR-383-5p, IBSP, RUNX2, FGF5 and BMP2K were observed. Alkaline phosphatase and Alizarin red staining were used to assess the improved osteogenic potential of H19-transfected P-MSCs. Results Transfection of lncRNA H19 downregulated the expression of miR-137-3p and miR-383-5p in human P-MSCs, and significantly upregulated the expression of RUNX2 and IBSP (P<0.05), and there were no significant differences in BMP2K and FGF5 expression between groups. Furthermore, osteogenic performance was enhanced in P-MSCs transfected with H19. Conclusion Transfection of lncRNA H19 in human placenta-derived mesenchymal stem cells can promote the expression of certain osteogenic and angiogenic factors, and improve the osteogenic  performance, which provides a possibility for the development of tissue-engineered bone with good osteogenic properties in the future.

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