Journal of Tissue Engineering and Reconstructive Surgery ›› 2025, Vol. 21 ›› Issue (1): 14-.

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Expression of TLR4/NF-κB signaling pathway in scar cancer tissues and effect of TAK-242 on scar cancer cells

  

  • Online:2025-02-25 Published:2025-03-06

Abstract:

Objective To analyze the expression of Toll like receptor 4(TLR4) and nuclear factor kappa B (NF-κB) in scar cancer tissues, and to investigate the effect of TLR4 inhibitor TAK-242 on scar cancer cells. Methods 20 cases of normal skin tissue, 20 cases of hypertrophic scar tissue, and 20 cases of scar cancer tissue were collected. Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of TLR4 and NF-κB protein and mRNA. The relationship between the expression of TLR4, NF-κB and clinical pathological factors such as patient gender, age, and tumor differentiation degree was analyzed. Scar cancer cells were cultured in vitro and treated with TLR4 inhibitor TAK-242. Cell proliferation activity was detected by MTT assay, and the expression of TLR4, NF- κB, MMP9, and TGF-β1 proteins was detected by Western blot assay. Results Compared with the control group, the expression of TLR4, NF-κB protein and mRNA in scar tissue and scar cancer tissue was significantly increased(P< 0.05); Compared with scar tissue, the expression of TLR4, NF-κB protein and mRNA in scar cancer tissue was significantly increased (P<0.05). The expression of TLR4 and NF- κB in scar cancer tissue was related to the degree of tumor differentiation (P<0.05). After intervention with TLR4 inhibitor TAK-242, the proliferation activity of scar cancer cells was  significantly reduced (P<0.05), and the protein expression of TLR4, NF- κB, MMP9, and TGF-β1 was downregulated (P< 0.05). Conclusion The TLR4/NF-κB signaling pathway is involved in the pathological process of scar tissue carcinogenesis, and the TLR4 inhibitor TAK-242 can inhibit scar cancer proliferation, which is expected to become a new target for scar tissue treatment.

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