Abstract:

Chronic wounds have emerged as a global public health concern， characterized by prolonged disease course， poor prognosis， and significant patient healthcare burden. Debridement， as a critical step in wound bed preparation， directly determines therapeutic outcomes. Enzymatic debridement refers to the selective application of enzymatic agents to necrotic tissue for targeted debridement. Among these， collagenase-based preparations are the most widely used and evidencesupported enzymatic debridement agents in clinical practice. They selectively degrade necrotic collagen without damaging healthy tissue， while also exerting multiple effects such as anti-inflammatory action， modulation of the wound microenvironment， promotion of granulation tissue formation， and enhancement of epithelialization. This article systematically reviews the historical development of enzymatic debridement， summarizes the mechanisms of collagenasebased preparations， and evaluates their clinical applications in burn wounds， diabetic foot ulcers， pressure ulcers， and vascular occlusive wounds. Furthermore， it provides insights based on the latest guidelines and research advancements to support standardized clinical practice.

Key words: Enzymatic debridement, &emsp, Collagenase-based preparations, &emsp, Wound healing, &emsp, Burns, &emsp, Diabetic foot ulcers, &emsp, Pressure ulcers, &emsp, Vascular occlusive wounds, &emsp, Wound microenvironment