Original article

Effectiveness of BK Channel Inhibitor on Proliferation,Apoptosis and Cytokines of Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton's Jelly

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  • Department of Nephrology,Shanghai Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China)

Received date: 2018-11-20

  Revised date: 2019-01-07

  Online published: 2019-02-20

Abstract

Objective To explore the effects of BK channels inhibitors on cell activity,apoptosis and cytokine secretion of mesenchymal stem cells derived from human umbilical cord Wharton's jelly(WJ-MSC).Methods The WJ-MSC were isolated and cultured by tissue adherent method.The expression of BK channels was detected by Western-blot.After treated with different concentrations of BK channels inhibitors,the activity of cells was detected by CCK-8,the apoptosis of cells was detected by Annexin-V-FITC/PI,and the expression of cytokine(IL6,IL10)was detected by Elisa.Results BK channels expression was found in WJ-MSC.BK channels inhibitors reduced cell activity,promoted cell apoptosis and secretion of cytokine(IL6),all of which have statistical significance(P<0.05).But BK channels inhibitors did not increase the secretion of IL-10.Conclusion BK channels inhibitors can inhibit the activity of WJ-MSC,promote cell apoptosis and promote the secretion of IL6.

Cite this article

SONG Ahui,TONG Yan,LIU Yingli . Effectiveness of BK Channel Inhibitor on Proliferation,Apoptosis and Cytokines of Mesenchymal Stem Cells Derived from Human Umbilical Cord Wharton's Jelly[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2019 , 15(1) : 5 -9 . DOI: 10.3969/j.issn.1673-0364.2019.01.002

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