Objective To investigate the culture system that can control the culture pressure and realize the dynamic culture of tissue engineered biofilm. Methods According to the basic principle of Pascal's law, a control method of media pressure is designed, and a tissue engineered biofilm dynamic culture system is established. The system is composed of culture dish, peristaltic pump, pressure control module, main controller and detection module. Results This system can realize the pressure control of the culture with a pressure range of 0.12-19.76 mmHg. Conclusion This system provides the pressure environment of biofilm in suitable pressure range required for culture. It provides a new method for tissue engineered biofilm dynamic culture technology.