Biodegradable scaffolds as one of the three main factors in tissue engineering, play an important role in tissue regeneration. An ideal scaffold material must have appropriate degradation rate that could match the forming rate of tissue regeneration process. However, how to monitor the degradation of scaffold materials has been a great challenge in this field. In this paper, the latest literature about monitoring of scaffold degradation in tissue engineering was reviewed. The principle of each method as well as their advantages and disadvantages were summarized and the potential methods were discussed.