Original article

Construction of Penis-Shaped Cartilage Using Tissue Engineering and 3D Printing Technologies

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  • 1 Plastic Surgery Research Institute, Weifang Medical College, Weifang 261042, China, 2 Department of Plastic and Reconstructive Surgery; Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People 's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, Chino, 3 National Tissue Engineering Center of China, Shanghai 200241, China.)

Online published: 2020-07-23

Abstract

Objective To explore the feasibility of constructing a penis-shaped cartilage using tissue engineering and 3D printing technologies. Methods A composite scaffold composed of PCL/HA and PGA/PLA were prepared for the reconstruction of penis-shaped cartilage by 3D printing. The penis-shaped PCL/HA scaffold prepared by 3D printing was beset between two separate PGA/PLA scaffolds that have a penis-shaped appearance. Articular chondrocytes were obtained from one-day-old roasters and then proliferated in order to meet the need of seeding sufficient cell into grafts. Cell-seeded constructs were cultivated in cartilage-inducing media for 4 weeks and then implanted under the dorsum skin of athymic nude mice. After 8 weeks' cultivation in vivo, constructs were harvested and grossly observed. Then the specimens were sagittally dissected to observe the interface between the regenerated cartilage and the porous PCL/HA scaffold and histology of engineered cartilage was examined in vivo. Results Penis-shaped models made of PCL/HA were printed into an exact penis shape and then composite scaffolds were assembled into a verisimilar penis. In the process of reconstruction, the initial shape of cell-seeded constructs were generally maintained. The surface of the specimens was smooth and continuous and no vascularization was observed. In cross section, the PCL/HA core were completely surrounded by the homogeneous neocartilage and the two phase interlocked well with each other. Conclusion It is feasible to construct a penis-shaped cartilage with the PCL/HA core surrounded by the homogeneous neocartilage by using tissue engineering and 3D printing technologies.

Cite this article

YUA N Zhaoyuan,NING Jin, CHEN Jie, XIA Huitang, TA O Ran, ZHOU Guangdong, XIA O Kaiyan, CA O Yilin . Construction of Penis-Shaped Cartilage Using Tissue Engineering and 3D Printing Technologies[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2017 , 13(3) : 123 -142 . DOI: 10.3969/j.issn.1673-0364.2017.03.002

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