Human urine-derived stem cells (hUSC) have been reported, and regarded as a candidate for seed cells in tissue engineering, which are highly expandable, and have self-renewal capacity, multi-differentiation potential and paracrine properties. More importantly, hUSC can be obtained via a non-invasive, simple way, and are low immunogenic without medical ethics restrictions. hUSC as an ideal source of stem cells, have demonstrated a prosperous progress in scientific research and potential application in the field of tissue engineering and regenerative medicine. The research achievement in urinary system, bone tissue engineering also implies potential applications of hUSC in oral maxillofacial tissue regeneration. In this paper, the application of hUSC in oral tissue engineering and regeneration were reviewed by summarizing domestic and overseas research progress on hUSC.