Objective To observe the role of subcutaneous pretreated bladder acellular matrix graft-silk fibroin (BAMG-SF) for bladder reconstruction in a rat bladder augmentation model. Methods After BAMG-SF was implanted subcutaneously for 1 day, 3 days, 7 days and 14 days in 4 four-week-old female Sprague Dawley rat, the scaffold and tissues around it were harvested for histology evaluation. Set 4 groups (n=3): simple BAMG-SF (control group), BAMG-SF 3 days subcutaneous precondition (3 days group), BAMG-SF 7 days subcutaneous precondition (7 days group) and the autologous tissue around BAMG-SF 7 days subcutaneous precondition (autologous group). All groups were used to reconstruct bladder in a rat bladder augmentation model, the bladder was harvested for histology and immunology evaluation 4 weeks later. Results After BAMG-SF implanted subcutaneously for 7 days, a large number of cells were observed in BAMG-SF. After BAMG-SF implanted subcutaneously for 14 days, BAMG-SF was covered by autologous tissue. The reconstruction of vascular and muscle in 3 days group were better than other groups, only the autologous group had no stone found. Conclusion BAMG- SF can be used for bladder repair, 3-day group has the best effect on bladder reconstruction and autologous group can avoid stone formation effectively.
WANG Qiong,XIAO Dongdong,ZHAO Yang,ZHOU Zhe,ZHOU Juan,WANG Zhong,ZHANG Ming,LU Mujun
. A Study on the Repair of Bladder by Pro-Producing Bladder AceHular Matrix Graft-Silk Fibroin in Rats[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2016
, 12(6)
: 335
-339
.
DOI: 10.3969/j.issn.1673-0364.2016.06.002