Objective To explore the technique of vagina reconstruction using pedicled transplants of sigmoid colon and its effect. Methods From February 2009 to September 2014, 13 female patients with congenital absence of vagina were treated with this technique in our hospital. The physical examination, questionnaire of satisfaction were performed after surgery. Results The operations were successfully completed in all the 13 cases. The appearance of cunnus was fine, the neovaginal length was 10-12 em, and the diameter was about 3 cm. The reconstructed vaginal wall was soft, moist and elastic. All the patients were followed up for 8 to 75 months. Ten patients had sexual life after operation. All the patients were satisfied with the results of the surgery. Conclusion The use of pedicled transplants of sigmoid colon for vaginal reconstruction is an effective method for female patients with congenital absence of vagina.