Objective To compare the percentage volume maintenance after the first and second autologous fat graft, and to explore the change of percentage volume maintenance. Methods From March 2011 to December 2014, 9 patients received autologous fat graft for breast augmentation were included for this retrospective study. The percentage volume maintenance after the first fat graft session and overall percentage volume maintenance after the second fat graft session were calculated using three-dimensional photography. The t test was performed to compare the results. Results The mean percentage volume maintenance was 0.25 ±0.06 after the first fat graft session, while the overall mean percentage volume maintenance was 0.32±0.06 after the second fat graft session. The percentage volume maintenance after the second fat graft session was significantly increased （P=0.000 7）. Conclusion There is a significant improvement of percentage volume maintenance after the second fat graft session.