Objective To obtain the morphology and three-dimensional structure characteristics of goat spinal dural and provide information for reconstructing artificial spinal dural. Methods The specimens of spinal dural were obtained to observe the microstructure, surface morphology, histology, collagen fiber diameter, distribution and distribution model through naked eyes, light microscope, scanning electron microscope （SEM） and transmission electron microscope （TEM）. Results The spinal dura looked like a milky white, translucent, flexible and elastic membrane. It was mainly composed of oriented collagen fibers while fibroblasts line sparsely along those fibers. Its inner and outer surfaces looked like mountain peaks through SEM observation. It was revealed by TEM that collagen fibers distribute orderly with an average diameter of （627±60） nm. The diameters of collagen fibers exhibited a bimodal distribution. Conclusion The goat spinal dura is a translucent membrane which is mainly composed of nanoscale oriented collagen fibers and sparsely scattered fibroblasts.