Amniotic fluid-derived stem cell is in a medium state between embryonic stem cell and adult stem cell, maintaining undifferentiated property. As not tumorigenic, its stern cell potentiality and immunomodulator properties make it an attractive candidate for cell therapy approaches. Till now, documented reports on the cell origin, characteristics, osteogenic differentiation, cuhivation and its tissue engineering application have been raised, regarding the cell lineage as a reasonable source for bone-defect reconstruction. Hereby, a review paper is made to conclude research progress on osteogenic differentiation of amniotic fluid-derived stem cell.