The use of stem cells in the treatment of myocardial infarct has been strongly pursued in recent years. Due to their availability, feasibility, plasticity, and their potential to differentiate into cardiomyocytes at specific media, adipose-derived stem cells （ASCs） have become an ideal source of donor cells. A significant effort has gone into elucidating which factors and techniques influence this differentiation. The methods and mechanisms of differentiation of ASCs into cardiomyocytes were reviewed in this paper.