Original article

Screening of siRNA Targeted PAR-2 in Keratinocyte

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  • Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China.

Online published: 2020-07-23

Abstract

Objective To design and screen small interfering RNA(siRNA) targeted protease-activated receptor-2(PAR-2) mRNA in Keratinocyte that can specifically and effectively suppress PAR-2 expression.Methods Three pairs of PAR-2 specific double stranded siRNAs were designed according to the sequence of PAR-2 mRNA.These siRNA were transfected into human keratinocyte by Lipofectamine 2000.The transcription level of PAR-2 was analyzed by semi-quantitative RT-PCR and PAR-2 protein expression was tested by western blot in order to evaluate the effect of RNA interference.Results Two of the three PAR-2 siRNA effectively inhibited the expression of PAR-2 and the second one is more effective.Conclusion PAR-2 siRNAs can be successfully designed and synthesized,which can specifically and effectively suppresses the expression of PAR-2 gene.The results in this study lay the foundation for further study on PAR-2 gene silencing by RNA interfere(RNAi).

Cite this article

YUAN Lei,JIN Rong,ZHANG Yan,ZHANG Yuguang . Screening of siRNA Targeted PAR-2 in Keratinocyte[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2012 , 8(2) : 69 -72 . DOI: 10.3969/j.issn.1673-0364.2012.02.003

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