Objective To investigate the effects of adipose derived stem cells(ADSC) on promoting lymphatic regeneration of lymphedematous mice tail.Methods ADSCs were isolated from the fat pad of the GFP transgenic mice and cultured to the second passage.Thirty days later,the ADSCs of the second passage were transplanted to the obstructed regions of the lymphedematous mice tail and the transplantation was repeated every seven days.The circumstance of tail were measured every third days and the lymph flow pattern was detected through micro-lymphangiography.Results ADSCs could participate in the formation of the crude pre-lymphatic fluid channels but no significant difference was observed on the reduction of the mice tail lymphedema.Conclusion The ADSCs could participate in the formation of pre-lymphatic fluid channels on the obstructive lymphatic regions,but there was no obvious effects on improving lymph flow.