Objective To explore the effects of single or combined application of VEGF and bFGF via the noninvasive medication of ultrasound-mediated transdermal drug transport(UMTDT) in the survival rate and microcirculation of fasciocutaneous flap with big ratio of length to width.Methods A total of 100 fascio-cutaneous flaps were performed on the sides of the back in ten pigs.The flap area ranged from 3 cm×15 cm.These flaps were randomly divided into 5 groups as follows. A:control group with blank ultrasound;B:VEGF by low-frequency UMTDT;C:bFGF by low-frequency UMTDT;D:VEGF and bFGF by localized injection in multiple points;E:VEGF and bFGF by low-frequency UMTDT.The quantitative analysis of the vitality of the flaps,capillary intensity and expression index were observed 10 days after operation.Results There was significant difference between the group of combined medication of both and bFGF via UMTDT and the other four groups in flap survival rate,capillary intensity and expression index.Conclusion The combined medication of VEGF and bFGF via UMTDT can significantly enhance the survival rate of fascio-cutaneous flaps with big ratio of length to width.