Journal of Tissue Engineering and Reconstructive Surgery >

2009 , Vol. 5 >Issue 1: 7 - 11

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.01.003

Original article

Expression of 3β-Hydroxysteroid Dehydrogenase in Rat Leydig Cells Cultured by Differential Adhesion In Vitro

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  • 1 Department of Plastic Surgery;Changhai Hospital;Second Military Medical University;Shanghai 200433;China. 2 Shanghai Laboratory of Tissue Engineering;Shanghai Ninth People′s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China.;

Online published: 2020-07-23

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Abstract

Objective To investigate the constitution of cells population, changes in 3β-hydroxysteroid dehydrogenase (3β-HSD) expression and androgen productivity in rat Leydig cells cultured by differential adhesion during 4 days primary culture. Methods Testes from 7 days and 3 weeks old rats were harvested, then Leydig cells were isolated by using collagenase dispersion, stainless steel mesh infiltration and differential adhesion. 3β-HSD expression in cultured cells was observed by immunohistochemistry and flow Cyt...

Key words： Leydig Cell; Differential Adhesion; Testosterone; 3β-hydroxysteroid dehydrogenase; Tissue Engineering

Cite this article

BI Hongda,WANG Xiaoyun,ZHOU Guangdongi,LIU Wei,LI Ming,XING Xin . Expression of 3β-Hydroxysteroid Dehydrogenase in Rat Leydig Cells Cultured by Differential Adhesion In Vitro[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009 , 5(1) : 7 -11 . DOI: 10.3969/j.issn.1673-0364.2009.01.003

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