Journal of Tissue Engineering and Reconstructive Surgery >

2009 , Vol. 5 >Issue 2: 61 - 64

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.04.001

Original article

Platelet Lysates Enhance the Proliferation of Human Bone-Marrow Stromal Cells In Vitro

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  • Institute of Radiation and Irradiation Medicine;Academy of Military Medical Science;Beijing 100850;China;

Online published: 2020-07-23

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Abstract

Objective To investigate the suitability and proliferation capacity of human platelet lysate in the production of bone-marrow stromal cells.Methods Human platelet lysates(PL) were prepared by freezing and thawing the platelet rich plasma.Marrow stromal cells(MSCs) were cultured in media containing PL and their proliferation status was evaluated by MTT assay.Furthermore,cell phenotypic characteristics were analyzed by flow cytometry and the differentiation along adipogenic and osteogenic pathways were assess...

Key words： Bone marrow stromal cells; Platelet; Cell culture; Proliferation

Cite this article

LOU Xiao,WANG Jinsong,JIN Jide,HE Ziming,WU Zuze,GUO Zikuan . Platelet Lysates Enhance the Proliferation of Human Bone-Marrow Stromal Cells In Vitro[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009 , 5(2) : 61 -64 . DOI: 10.3969/j.issn.1673-0364.2009.04.001

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