Journal of Tissue Engineering and Reconstructive Surgery >

2009 , Vol. 5 >Issue 2: 65 - 69

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.04.002

Original article

VEGF Gene Transfected Human BMSCs Acting as Seeding Cell for Tissue Engineering Bond in Orbital Wall Bone Defects

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  • Department of Ophthalmology;Shanghai Ninth People s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China;

Online published: 2020-07-23

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Abstract

Objective To investigate the effect VEGF-gene transfected BMSCs to construct tissue engineered bone in the treatment of orbital wall bone defects.Methods In vitro isolated and expanded BMSCs of passage 2 were transfected with adenoviruses containing human VEGF gene,and the target gene expression was evaluated by Real-time PCR and Western Blot.BMSCs were harvested and seeded into the coral scaffolds to construct tissue engineered bone.VEGF protein secretion from the cells was detected using Elisa method.Bila...

Key words： Vascular endothelial growth factor; Bone marrow stromal cells; Tissue engineered bone; Vascularity; Orbital wall bone defect

Cite this article

XIAO Caiwen,FAN Xianqun,ZHOU Huifang,BI Xiaoping,SHEN Qin,WANG Yefei . VEGF Gene Transfected Human BMSCs Acting as Seeding Cell for Tissue Engineering Bond in Orbital Wall Bone Defects[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009 , 5(2) : 65 -69 . DOI: 10.3969/j.issn.1673-0364.2009.04.002

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