Journal of Tissue Engineering and Reconstructive Surgery >

2009 , Vol. 5 >Issue 2: 75 - 78

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.04.004

Original article

Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene

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  • 1 Second Department of Cardiology;Southern building;General Hospital of PLA;Beijing 100853;China.2 Department of Experimental hematology;Institute of Radiation Medicine;Academy of Military Medical Sciences;Beijing 100850;China;

Online published: 2020-07-23

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Abstract

Objective To construct recombinant retroviral vector carrying firefly luciferase reporter gene(fluc) for further study of bioluminescence imaging in vivo.Methods Target gene-fluc was obtained by cutting firefly luciferase reporter gene(fluc) vector pGL3-Basic using restriction enzyme digestion.Then fluc gene was sub-cloned into retroviral vector pLXSN.GP+E86 and PA317 pakage cells were infected by the obtained recombinant retroviral vector pL(fluc)SN with ping-pong infection assay and screened with G418 res...

Key words： Firefly luciferase reporter gene（fluc） vector; pGL3-Basic; Recombinant retroviral vector; pLXSN; Construction

Cite this article

BIAN Suyan,GAI Luyue,YE Ping,YANG Yuefeng,WANG Rongliang,WANG Hua,GUO Zikuan,Wang Lisheng . Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009 , 5(2) : 75 -78 . DOI: 10.3969/j.issn.1673-0364.2009.04.004

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