Original article

A Preliminary Experimental Study on Repairing Defects of Extremities Using Tissue Engineer Bone

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  • 1 Department of Plastic and Reconstructive Surgery;Shanghai Ninth People s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China.2 Department of orthopedics;Hangzhou First People s Hospital;Hangzhou 310006;China.;

Online published: 2020-07-23

Abstract

Objective To explore the feasibility of human bone marrow stromal cells(hBMSCs) and its clinical repair of limb bone defects.Methods Nine patients of bone defects(5 bone tumors,4 bone defects) were included in this study.The hBMSCs were isolated by Percoll gradient centrifugation with bone marrow aspirated from iliac crest of each patient.hBMSCs were cultured in vitro and induced to become osteogenic cells in the DEME medium containing 10% self-serum,β-glycerophosphate(10 nmol/L) dexamethasone(10-8 mol/L),L...

Cite this article

ZHU Lian,ZOU Cheng,ZHU Min,CUI Lei,CAO Yilin . A Preliminary Experimental Study on Repairing Defects of Extremities Using Tissue Engineer Bone[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009 , 5(3) : 121 -124 . DOI: 10.3969/j.issn.1673-0364.2009.06.001

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