抗草甘膦转基因大豆的获得

doi:10.3969/J.ISSN.1671-9964.2016.01.001

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摘要

大豆[Glycine max (L.) Merrill]是我国主要的粮食和油料作物, 但近年来由于大量进口美国的转基因大豆, 使我国的大豆生产严重萎缩。生物技术方法应用于改良大豆的农艺性状和产品质量以及对除草剂的耐受性, 特别是对草甘膦的耐受性这一个重要性状。本研究以东农50为受体, 采用农杆菌介导法, 将抗草甘膦基因G10-EPSPS基因转入到大豆中, 先后获得了3株T₀代植株, 在T₀代进行抗性鉴定后, 1株得到种子。试验通过对转基因后代的PCR检测、Western检测以及草甘膦抗性鉴定, 证明外源基因已整合到植物基因组中, 并在T₀、T₁和T₂代稳定表达。本研究为转基因大豆育种提供了材料和数据。

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	崔云云
	曹越平

关键词 ：大豆, 草甘膦, 转基因, 分子检测, 抗性

Abstract：

Soybean (Glycine Max L.Merrill) is an important grain and oil crop in China.However its production has been seriously reduced due to large scale soybean import from US Methods of biotechnology have been applied to soybean for improvement of the agronomic traits and the quality of the product.One such agronomic trait important in soybean production is herbicide tolerance, in particular, tolerance to glyphosate herbicide.In this study, Agrobacterium-mediated method was used to introduce G10-EPSPS into soybean Dongnong 50.Three transgenic soybean plants were obtained.The obtained transgenic soybean plants were all resistant to glyphosate and 1 event expressed the resistance stably in T₀, T₁and T₂ generations.Molecular analysis by PCR and Western blot verified that G10-EPSPS was integrated into the soybean genome and inherited stably in T₀, T₁and T₂ generations.It would be helpful for further study and soybean breeding.

Key words： soybean glyphosate transformation molecular detection resistant

收稿日期: 2015-02-23 出版日期: 2016-03-08

基金资助:国家转基因重大专项(2013ZX08004001-004)

作者简介:

作者简介: 崔云云(1988-), 女, 硕士生, 研究方向:大豆遗传育种, E-mail: kxmami@163.com;

通讯作者: 曹越平(1967-), 女, 博士, 教授, 博士导师, 研究方向:大豆遗传育种, E-mail:yuepingcao@sjtu.edu.cn

引用本文:

崔云云, 曹越平. 抗草甘膦转基因大豆的获得[J]. 上海交通大学学报(农业科学版), 2016, 34(1): 1-4.
Yun-yun CUI, Yue-ping CAO. Obtaining of Transgenic Glyphosate-Resistant Soybean. Journal of Shanghai Jiaotong University (Agricultural Sciences), 2016, 34(1): 1-4.

链接本文:

http://www.qk.sjtu.edu.cn/jsjtua/CN/10.3969/J.ISSN.1671-9964.2016.01.001 或 http://www.qk.sjtu.edu.cn/jsjtua/CN/Y2016/V34/I1/1

Fig.1 大豆转化的部分转化时期

注: A:共培养时期; B:诱导时期; C和D:伸长时期; E移栽时期

Fig.2 T₀代PCR检测

注: M: Marker DL2000; 1、A、A1、A2:T₀代植株; CK+:阳性对照; CK-:阴性对照

Fig.3 成熟期T₁代植株

Fig.4 植株不同器官Western blot 检测结果

注: M: Marker; 1:阴性对照; 2:根部; 3:茎部; 4:叶; 5:花; 6豆荚; 7:阳性对照

Fig.5 部分转基因T₂代植株草甘膦抗性鉴定

注:A、B、C:转基因植株; CK:对照植株

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