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| Effectof Taurine on Development and Implantation of Kunming Mouse Early Embryos |
Jing-jing FAN1,2( ), Qiu-kai ZHENG3 |
1. Biological and Agricultural Engineering College ofWeifang University, Weifang 261061, China
2. Key Laboratory of Biochemistry & Molecular Biology in Universities of Shandong, Weifang University, Weifang 261061 China
3. College of Chemistry and Chemical Engineering, Weifang University, Weifang 261061, China |
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Abstract The effects of taurine on development and implantation of Kunming mouse early embryos were investigated.The embryos were cultivated in modified potassium simplex optimized medium(mKSOM)with different concentration of taurine.The percentage of 4-cell, blastocysts and average total cell number of blastocysts were calculated after 24 h and 72 h.Cornua uteri injection technique was used to investigate the effects of β-alaline(β-ala, taurine transport inhibitor)on embryo implantation.The results showed that there were significant differences between 10 mmol/L taurine treatment and control in the percentage of 4-cell(P<0.05), blastocysts and total cell number of blastocysts(P<0.01).Meanwhile, injection of β-ala(20 μg/mL)into uterine horn significantly reduced the number of implanted embryos on D9.This study explicated the effects of taurine on the development and implantation of early Kunming mouse embryos, providing a useful reference for further uncovering of mechanism of embryo development and implantation.
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Received: 01 February 2015
Published: 08 March 2016
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Effect of taurine on mouse embryo blastocyst ratio in vitro
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牛磺酸/
(mmol·L-1)
Tau | β-丙氨酸/
(mmol·L-1)
β-ala | 2-细胞胚胎数
No. of 2-细胞 | 胚胎数 No.of embryos | | | 囊胚细胞数
No. of cell
(Mean±SE) | 囊胚细胞数 范围
Range | 发育至4细胞个数(比例)
No.
of 4-cell(ratio) | 发育至囊胚个数
(比例)No.
of blastocysts(ratio) | | | - | - | 435 | 338(77%) | 270(62%) | 39±2.18 | 27-66 | | 5 | - | 293 | 219(75%) | 187(64%) | 40±2.13 | 23-68 | | 10 | - | 376 | 327(87%)* | 300(80%)** | 50±1.84** | 25-83 | | - | 10 | 408 | 261(64%) | 204(58%) | 35±2.55 | 23-52 | | 10 | 10 | 312 | 221(71%) | 184(59%) | 41±3.36 | 29-67 |
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Effect of taurine on the development of KM mouse embryos in mKSOM
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Fluoresence staining of mouse blastocysts in mKSOM mediums containing different concentration of taurine
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Effect of taurine added to mKSOM on mouse embryo development in vitro
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Effect of intrauterine injection of β-alanine at different concentrations in uterus on D4 of pregnancy
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| 孕D9胚胎数No. of embryos on D9(Mean±SE) | 生理盐水
Normal saline | β-丙氨酸(20 μg·mL-1)
β-ala | β-丙氨酸(200 μg·mL-1)
β-ala | | 11.67±0.67 | 左侧Left | 12.66±0.89 | 11.00±0.58 | | 1.67±0.63** | 右侧Right | 10.67±0.43 | 4.67±0.55** |
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The number of embryos after intrauterine injection of β-alanine on D9 of pregnancy
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2016, Vol. 34 
