HighlightsMore...

01 August 2023, Volume 19 Issue 4 Previous Issue   
Curcumin-loaded nanofilm stabilize in vivo chondrogenesis of stem cellengineered cartilage using an encapsulation model
HU Doudou, XU Yong, JIA Wei
2023, 19 (4):  329-335. 
Abstract ( 8 )   PDF (1500KB) ( 8 )  
Objective　To explore the feasibility of a curcumin-loaded nanofilm using an encapsulation model to stabilize in
vivo chondrogenesis of stem cell-engineered cartilage. Methods　Curcumin (CUR) with anti-angiogenic activities was loaded
into poly(L-lactide-co-caprolactone) (PLCL) and underwent electrospun process to produce a CUR/PLCL nanofilm, and a PLCL
nanofilm was identically prepared as a control group. The morphologies of PLCL and CUR/PLCL nanofilms were observed using
SLR camera and scanning electron microscope. The anti-angiogenic activity of CUR/PLCL nanofilm was evaluated through the
in vitro co-culture with human umbilical vein endothelial cells for 6 hours. In addition, rabbit-derived bone marrow stem cells
(BMSCs) were seeded into a porous gelatin scaffold and underwent chondrogenic culture for 3 weeks. The BMSC engineered cartilage was evaluated using SLR camera and histological staining. Finally, the BMSC engineered cartilage was embedded using 
the CUR/PLCL nanofilm and implanted into nude mice subcutaneously for 6 weeks. The achieved samples were evaluated using
SLR camera, micro-CT, and histological staining examinations, aiming to distinguish chondrogenesis and ossification conditions.
Results　In contrast to the white PLCL nanofilm, the CUR/PLCL nanofilm showed yellow color, whereas both nanofilms exhibited similar nano-scaled structure. The in vitro tube formation assay suggested that the CUR/PLCL nanofilm possessed enhanced
anti-angiogenic activity than PLCL nanofilm. A BMSC engineered cartilage tissue was successfully achieved via in vitro chon
drogenic culture of the BMSC-gelatin construct for 3 weeks, as evidenced by cartilage-like appearance in photograph, typical
lacunar structure and cartilage-specific extracellular matrix formation in HE staining, and positive stained glycosaminoglycan in
safranin-O staining. After subcutaneously implanted into nude mice for 6 weeks, the gross images indicated that the PLCL group
was obvious vascularized whereas the CUR/PLCL group was absent from vascular invasion. The micro-CT images suggested that
the PLCL group exhibited abundant bone trabecular structure but not in the CUR/PLCL group. The HE and saf-O/FG staining
further confirmed that the PLCL group showed typical bone trabecular structure and bone-specific matrix, and the CUR/PLCL
group displayed typical mature lacuna and cartilage-specific extracellular matrix. Conclusion　CUR/PCL nanofilm could stabilize in vivo chondrogenesis of stem cell-engineered cartilage using an encapsulation model.
Related Articles | Metrics

In-situ bioprinting and hair follicle reconstruction of full-layer skin injury

LI Xingzhou, CHEN Xiaojun, SUN Mengzhe, et al
2023, 19 (4):  336. 
Abstract ( 13 )   PDF (1132KB) ( 5 )  
Objective　Using in-situ bioprinting to repair full-thickness skin wound on the back of nude mice to achieve
in-situ repairing and hair follicle reconstruction. Methods　Dorsal full-thickness skin defects (7 mm in diameter) was created
and covered with CA/BC in nude mice. About 5×105 primary cells from neonatal C57 mice skin were used as seed cells and
transplanted to the wound: 10 points of high-concentration cell plotting was printed in the CA/BC via in-situ bioprinting in ex
perimental group while 1 mL injector was used manually in control group. After 4 weeks, wound repairing and hair growth were
observed, and the structure of new hair follicles was examined by H&E staining. Results　The skin defect wound of the exper
imental group recovered well with vigorous and uniform hair growth, while the hair of the control group was sparse, uneven or
even no hair growth. HE staining showed that there was complete hair follicle structure in the new skin of the experimental group,
while there was almost no hair follicle formation in the control group. Conclusion　The feasibility of in-situ bioprinting tech
nology in skin defect repair and hair follicle reconstruction is preliminarily verified.
Related Articles | Metrics

 Mechanism research of adipose-derived mesenchymal stem cell-derived exosomes in wound healing of full-thickness skin defect in rats

FU Wen, WANG Xiangchen, WANG Yangui, et al
2023, 19 (4):  342. 
Abstract ( 10 )   PDF (2604KB) ( 8 )  
Objective　To explore the mechanism of adipose-derived mesenchymal stem cell-derived exosomes (hADSC-Exo) on wound healing of full-thickness skin defect in rats. Methods　hADSC and hADSC-Exo were isolated and identified.
Human microvascular endothelial cells (hMECs) were divided into control group, positive control group (treated with 200 μg/L recombinant human epidermal growth factor) and hADSC-Exo group (treated with 100 μg/mL hADSC-Exo). The proliferation, migration and angiogenesis of hMECs were detected by CCK-8, scratch and angiogenesis experiments. A total of 60 rats were randomly divided into control group, positive control group, hADSC group and hADSC-Exo group, 15 rats in each group. Full-layer
skin defect wounds were prepared on the back of all rats. Each group was injected with 200 μL 1×PBS, 100 μg/kg recombinant
human epidermal growth factor, 200 μL 1×PBS containing 1×106 hADSC and 200 μL 1×PBS containing 200 μg hADSC-Exo, re
spectively. Three rats were randomly reserved in each group. The wound images were taken and the wound area was calculated
on the 0, 3, 7, 10 and 12 days respectively. The rest of the rats in each group were randomly divided into 4 groups, with 3 rats in
each group. The unhealed wound tissues were collected on the 3, 7, 10 and 12 days respectively, and tissue samples were prepared into paraffin sections. HE and Masson staining were used to observe the wound healing. The expression of proteins related
to inflammation and wound healing was detected by immunohistochemistry. Results　The results of cell experiment showed
that compared with the control group, the cell proliferation activity, cell migration rate, lumen length and the number of branching points increased in the positive control group and hADSC-Exo group (P<0.05). Compared with the positive control group,
the cell proliferation activity, cell migration rate, lumen length and the number of branching points in hADSC-Exo group were
further increased (P<0.05). The results of animal test showed that compared with the control group, the wound healing rate and
the expression levels of CD206, Collagen Ⅰ , Collagen Ⅲ and Aquaporin 3 (AQP3) in the positive control group, hADSC group
and hADSC-Exo group increased (P<0.05), while the expression levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6,
CD68, keratin 1 (KRT1) and the ratio of Collagen Ⅰ /Collagen Ⅲ decreased (P<0.05), the new epidermis was more intact and
thicker, the proliferation of fibroblasts and the deposition of new collagen in the wound were significantly increased. Compared
with the positive control group, the expression levels of CD206, Collagen Ⅰ , Collagen Ⅲ and AQP3 in hADSC group and hADSC-Exo group were further increased (P<0.05), while the expression levels of TNF-α, IL-6, CD68, KRT1 were further decreased
(P<0.05). Conclusion　hADSCs-Exos can enhance the proliferation, migration and angiogenesis of hMECs, reduce the level of
wound inflammation, promote the proliferation of wound cells, collagen remodeling, and accelerate wound healing, which has the
potential to be used as a treatment drug for skin wounds.
Related Articles | Metrics

 Preliminary screening and analysis of differentially expressed microRNAs during the repair of facial nerve injury

XU Wanlin, WU Yifan, YANG Wenjun
2023, 19 (4):  352. 
Abstract ( 10 )   PDF (1375KB) ( 6 )  
Objective　To screen differentially expressed microRNAs during the repair of facial nerve crush injury (3 d, 14
d) by high-throughput RNA sequencing technology, and to verify the expression of microRNAs of interest. Methods　The rat
model of facial nerve crush injury was established, and the process of nerve repair was evaluated by facial appearance observation and histological staining. Three samples were collected at 3 and 14 days after compression respectively, and microRNA
sequencing analysis was performed. Bioinformatics method was used to screen for microRNA with significant differences, and
qPCR was used to verify the expression of the 4 microRNAs that were most significantly down-regulated and up-regulated in
nerve samples at different periods. Results　The model of facial nerve injury was successfully created by crushing the facial
nerve trunk of rats. Facial paralysis appeared immediately after the operation. The facial appearance observation and histological
staining results all indicated that the facial nerve injury was repaired to a certain extent 14 days after the operation. The RNA
sequencing results showed that a total of 96 microRNAs were significantly up-regulated and 115 microRNAs were significantly
down-regulated. Four microRNAs with the most obvious up-regulation and down-regulation were selected for qPCR validation,
and the results showed consistency between their expression and the trend of sequence detection results. Compared to 3 days
after operation, the expression of miR-200a-3p was significantly up-regulated (P<0.01) and miR-300-5p was significantly
down-regulated (P<0.05) in the specimens 14 days after operation. Conclusion　The expression of microRNA shows different
characteristics at different stages after facial nerve injury. Abnormal expression of microRNAs (such as miR-200a-3p, miR-
300-5p) may provide new targets for the treatment of facial nerve injury.
Related Articles | Metrics

Akkermansia muciniphila outer membrane protein 1100 promotes wound healing in diabetic rats

LIN Weiyi, GE Chengwang, TANG Xiaowei, et al
2023, 19 (4):  358. 
Abstract ( 14 )   PDF (2047KB) ( 12 )  
Objective　To investigate the effects of Akkermansia muciniphila outer membrane protein 1100 (Amuc_1100)
on wound inflammation and collagen fiber synthesis in diabetic rats. Methods　Twenty male SD rats were induced into diabeticrats by intraperitoneal injection of streptozotocin. The defective wound model of full-thickness back skin was constructed and the
rats were divided into two groups, control group and Amuc_1100 group. The Amuc_1100 group was injected locally with 300 μL
Amuc_1100 solution with a concentration of 333 μg/mL, the control group was injected with the same amount of normal saline.
Wounds were photographed and the wound healing rate were compared on day 0, 3, 7, 10 and 14, respectively. Five rats in each
group were sacrificed and harvested on day 7 and 14. Hematoxylin-eosin (HE) staining was used to evaluate inflammatory cellinfiltration and histological morphology of the wound. Masson three-color staining was used to evaluate collagen fiber deposition.
The mRNA expression levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), collagen fiber
1A1 (Col1A1) and collagen fiber 3A1 (Col3A1) were compared by real-time quantitative polymerase chain reaction (RT-PCR).
Results　The wound healing rate in Amuc_1100 group was higher than that in control group on day 3, 7, 10 and 14 (P<0.05).
HE staining showed that the Amuc_1100 group had less inflammatory cells and more collagen fiber deposition in wound tissue.
Masson three-color staining showed that the collagen in the Amuc_1100 group was thicker, deeper dyed and more compact
than control group. RT-PCR showed that the relative mRNA expressions of TNF-α and IL-6 were significantly decreased in
Amuc_1100 group compared with the control group (P<0.05), and the relative mRNA expressions of IL-10, Col1A1 and Col3A1
were significantly increased compared with the control group (P<0.05). Conclusion　Amuc_1100 can improve the wound healing rate of diabetic rats, and the mechanism may be related to reducing local inflammatory response and increasing collagen fiber
synthesis.
Related Articles | Metrics

 Treatment of cicatricial cryptotia and utilization of para-auricular scar flap

DONG Liwei, TANG Yinke, CHU Feifei, et al
2023, 19 (4):  366. 
Abstract ( 12 )   PDF (1758KB) ( 4 )  
Objective　To explore the treatment of cicatricial cryptotia and utilization of para-auricular scar flap. Methods
　From 2014 to 2018, 12 cases of cicatricial cryptotia were treated. The correction of cicatricial cryptotia deformity was per
formed by releasing the superior auricular scar adhesion, correcting the deformed auricular cartilage, reshaping the antihelix.
A local scar flap was designed and transferred to reconstruct cranioauricular groove, and the skin defects of the superior- and
dorsal- part of the auricle were repaired as well. Results　All the cicatricial cryptotia were corrected by scar adhesion releasing,
deformed auricular cartilage remodeling and the utilization of a para-auricular scar flap. Follow-up ranged from 3 months to 4
years, and all the patients had significant improvement and stabilized appearance of the auricle. Conclusion　Scar adhesion
releasing, auricular cartilage remodeling and the para-auricular scar tissue repair could be served as the reliable method of de
formity correction, and significant improvement could be obtained without additional donor site compromise.
Related Articles | Metrics

 Application of anterograde and reverse dorsalis pedis skin flap for repairing defects in the lower leg and foot

DU Xuanyu, CHEN Yong, WANG Qian, et al
2023, 19 (4):  371. 
Abstract ( 14 )   PDF (1269KB) ( 13 )  
Objective　To explore the application of anterograde and reverse dorsalis pedis skin flap in the repair of the de
fects in the lower leg and foot. Methods　From Jan. 2018 to Dec. 2021, 14 cases of dorsalis pedis flap were used to repair the
defects in the lower leg and foot, including 9 cases of anterograde dorsalis pedis skin flap and 5 cases of reverse dorsalis pedis
skin flap. The donor site was repaired with skin graft directly or covered with artificial dermis followed by skin graft 2-3 weeks
later. Results　All flaps survived well with primary healing. Most of skin grafts survived and the residual wound healed with
dressing change. In the follow-up period, the appearance of the flap was satisfactory. The appearance of donor sites repaired
with artificial dermis and secondary skin graft were better than that repaired by skin graft only. Foot function recovered well.
Conclusion　Dorsalis pedis flap is a reliable choice for repairing defects in the lower leg and foot. The application of artificial
dermis before skin graft can obviously increase the survival rate and improve the long-term appearance of donor site.
Related Articles | Metrics
Clinical observation of the effect of JianpiQushi Fang on the treatment of keloid in patients with phlegm-damp/damp-heat TCM constitution
TANG Renyan, XIA Lingling, CAO Zhengdong, et al
2023, 19 (4):  376-379. 
Abstract ( 26 )   PDF (889KB) ( 8 )  
Objective　To explore the clinical effect of Traditional Chinese Medicine (TCM) formula “JianpiQushi Fang” in the treatment of keloid in patients with phlegm-damp/damp-heat TCM constitution, and to provide preliminary clinical evidence for TCM systemic treatment of keloid. Methods　Twenty-five keloid cases were diagnosed as phlegm-dampness/damp heat constitution by TCM constitution criteria. The patients were treated with“JianpiQushi” decoction with 1 dose daily (half dose twice a day) and continued for 4 weeks as a course of treatment. Physique score, local scar pain/pruritus symptoms, and Vancouver scar scale (VSS) score were compared before and after the treatment. Results　Compared with before treatment, the physique score, scar local pain score and pruritus symptom score were significantly improved after treatment (P<0.05), while the scar VSS score had no significant change (P>0.05). Conclusion　“JianpiQushi Fang” can effectively reduce the symptoms of keloid in patients with phlegm-damp/damp-heat TCM constitution, but its effect on improving the appearance and texture of scar is limited, suggesting that it should be combined with local surgical treatment.
Related Articles | Metrics

Effect of optic nerve decompression surgery in the treatment of traumatic optic neuropathy: A Meta-analysis

ZHAO Yan, ZHANG Yuanjie, GUO Zhilin
2023, 19 (4):  380. 
Abstract ( 11 )   PDF (1185KB) ( 4 )  
Objective　To systematically evaluate the efficacy of optic nerve decompression (OND) in the treatment of traumatic optic neuropathy (TON). Methods　A computer search was conducted for the retrospective studies on OND in the treatment of TON in PubMed, EMbase, The Cochrane Library and Web of Science from database establishment to February 7, 2023.
The obtained studies were independently screened and extracted by two researchers according to inclusion and exclusion criteria,
and their quality was assessed using the Newcastle-Ottawa Scale (NOS). The postoperative outcomes were considered to indicate
the efficiency of OND for TON. Interval estimates were expressed using a 95% confidence interval (CI), and the meta-analysis of
the collected data was performed using Stata 16.0 software. Results　A total of 42 studies, including 3,579 patients, were finally
included. The results of the meta-analysis were as follows: The overall efficiency of OND for TON was 54.2% (95% CI 49.2%-
59.2%). Subgroup analysis with different surgical approaches showed that the efficiency of endoscopic transorbital, transnasal,
and transcranial approaches for TON was 56.0% (95% CI 46.2%-65.7%), 52.8% (95% CI 45.1%-60.5%), and 50.0% (95%CI 36.1%-63.9%), respectively. The efficiency of OND ≤ 7 and >7 days post-injury was 69.5% (95% CI 55.0%-82.5%) and28.6% (95% CI 21.4%-36.2%), respectively, whereas 1-2 and 3-7 days post-injury, it was 71.5% (95% CI 45.2%-92.3%)and 51.2% (95% CI 65.3%-100.0%), respectively. The OND efficiency with and without light perception was 79.2% (95% CI 84.6%-100.0%) and 36.3% (95% CI 30.2%-42.6%), respectively. The overall efficiency of optic nerve sheath incision alongwith optic canal decompression was 40.7% (95% CI 27.0%-55.1%). Conclusion　OND is an effective method to improve vision in patients with TON. The difference in preoperative characteristics was not significant among different surgical methods. The transorbital approach showed the most favorable postoperative outcomes. The time of surgery should be as early as possible, and patients with preoperative light perception have a better prognosis. Also, intraoperative dissection of the optic nerve sheath may be helpful in vision restoration.
Related Articles | Metrics

 Curative effect and mid-term follow-up analysis of anterior cervical self- stabilizing cage in the treatment of double-segment cervical spondylotic myelopathy 

YANG Guangxu, SHI Fajian, WANG Haibin, et al
2023, 19 (4):  391. 
Abstract ( 8 )   PDF (1086KB) ( 4 )  
Objective　To investigate the efficacy and mid-term follow-up analysis of anterior cervical self-stabilizing cage in the treatment of double-segment cervical spondylotic myelopathy. Methods　Fifty patients with double-segment cervical spondylotic myelopathy who underwent anterior cervical discectomy and fusion in our hospital from January 2021 to December
2021 were selected, and they were randomly divided into the observation group and the control group, with 25 cases in each
group. The patients in the observation group were treated with anterior cervical approach zero-notch self-stabilizing interbody
cage, while the patients in the control group were treated with the traditional screw-plate system internal fixation cage. A 6-month
interim follow-up was performed postoperatively. The operation time, intraoperative blood loss, NDI score, JOA score, bone graft
fusion rate, neck VAS score, upper limb VAS score, neurological function improvement rate before operation, 3 months after operation and 6 months after operation were observed in the two groups. And cervical imaging indicators such as C2-7Cobb angle,
C2-7SVA, T1S and segmental angle were measured. The incidence of postoperative complications in the two groups was observed. Results　The operation time and intraoperative blood loss were significantly different between the two groups (P<0.05),
but there was no significant difference in hospitalization time between the two groups (P>0.05). The NDI score, JOA score, bone
graft fusion rate, neck VAS score, upper extremity VAS score and neurological function improvement rate of the two groups at
3-month and 6-month follow-up were all better than those before operation (P<0.05). However, there was no significant difference in preoperative, 3-month and 6-month follow-up (P>0.05). There was no significant difference in bone graft fusion ratebetween observation group and control group at each time point (P<0.05). The C2-7 Cobb angle, C2-7SVA, T1S and segmentalangle of the two groups at 3-month postoperative and mid-term 6-month follow-up were better than those before surgery (P<0.05).
However, there was no significant difference in preoperative, 3-month and 6-month follow-up (P>0.05). The incidence of postoperative dysphagia in the observation group was significantly lower than that in the control group (P<0.05), and there was no
significant difference in other postoperative complications between the two groups (P>0.05). Conclusion　The anterior cervical
self-stabilizing cage has good curative effect in the treatment of double-segment cervical spondylotic myelopathy, with short operation time and low incidence of postoperative dysphagia.
Related Articles | Metrics
Research on the application of 3D printing technology in personalized customization of neck functional stents for#br# scar patients#br#
NI Xiaojun, ZHUANG Leilan, SHEN Lei, et al
2023, 19 (4):  397. 
Abstract ( 7 )   PDF (826KB) ( 3 )  
Objective　To explore the application effect of 3D printing technology in personalized customization of neck functional stents for scar patients. Methods　Fifty patients who underwent neck scar treatment in our hospital from March 2021 to August 2021 were selected and randomly divided into two groups according to the random number table method. On the basis of conventional anti-scar drug treatment, the control group was treated with a conventional size neck brace for pressure treatment, andthe observation group was treated with a personalized neck functional stent made by 3D printing technology for pressure treatment.
The patients were followed up for 12 months and assessed by the Vancouver Scar Scale (VSS) and the Patient and Observer Scar
Assessment Scale (POSAS). Results　A total of 43 patients completed the whole course of treatment. The time for the observation group to reach the target pressure value was significantly shorter than the control group (P<0.05); All indicators of VSS scores
were better than the control group (P<0.05); Multiple indicators of POSAS scores were better than the control group (P<0.05).
Conclusion　Implementing 3D printing technology to customize personalized functional stents for neck scar patients is beneficial
for maintaining stable pressure treatment effects and improving scar treatment efficacy

Related Articles | Metrics
 Establishment and application of the patient safety smart warning system based on 5G Internet of things technology#br# in Plastic and Reconstructive Surgery nursing #br#
ZHANG Yongmei, WANG Ping, BIAN Weiwei, et al
2023, 19 (4):  402. 
Abstract ( 5 )   PDF (857KB) ( 6 )  
Objective　To develop the patient safety smart warning system based on 5G internet of things technology, and
to explore its application effect in the Plastic and Reconstructive Surgery nursing. Methods　The patient safety smart warning
system was constructed based on research group establishing, literature research and Delphi study, then patient safety warning
indicators were screened and modules were built. Based on 5G network, the patient safety indicators and the connected equipment involved were integrated with the system. Then the system was applied in plastic and reconstructive surgery ward after staff
training. Two months before and after the application of the system, the postoperative complications, incidence of adverse events
and discovery rate of potential complications in patients were calculated, and graded care quality was scored. Medical staff’s
application satisfaction was investigated using self-designed questionnaire. Results　After the application of the patient safety
smart warning system, the discovery rate of potential complications in patients was increased significantly (P<0.05). The incidence of postoperative complications and adverse events decreased with no significant difference (P>0.05). The score of graded
care quality improved from 97 to 99 after the application of the system. The overall satisfaction score of medical staff to patient
safety smart warning system was 4.59±0.49. Conclusion　Application of the patient safety smart warning system based on 5G
Internet of things technology can improve the discovery rate of potential complications in patients, enhance the predictability of 
patients’safety risks and the awareness of safety risk management of medical staff, promote the timely follow-up of clinical intervention measures, and ensure the safety of patients.
Related Articles | Metrics
New advances in the study of scaffold materials for cartilage tissue engineering
YUE Guangren, WANG Ximei, LI Yu
2023, 19 (4):  408-413. 
Abstract ( 14 )   PDF (875KB) ( 10 )  
Lacking blood vessels and nerve nourishment, cartilage has limited ability to repair and regenerate. Combining scaffolds, cells and active factors, tissue engineering has been considered as an important new direction for repairing cartilage injury. Scaffold plays an important role in the cartilage regeneration process, not only providing a support site for cell adhesion, but also improving the local microenvironment and affecting cell proliferation and differentiation. Many materials have been used to make biodegradable scaffolds: natural materials, synthetic materials and composite materials. In this paper, the materials that have been relatively widely used in cartilage tissue engineering were reviewed so as to provide valuable reference for researchers in this field.
Related Articles | Metrics
Research progress of NOTCH signaling pathway and endothelial-to-mesenchymal transition in infantile hemangioma
SUN Xiaoting, MA Xiaorong
2023, 19 (4):  414. 
Abstract ( 7 )   PDF (854KB) ( 7 )  
Infantile hemangioma (IH) is a benign tumor that occurs in infants and childhood. The pathogenesis of IH has
not been fully elucidated. Although the course of the disease is self-limiting, partial patients may have sequelae that affect the
aesthetics or normal function. NOTCH signaling is an evolutionarily conserved signaling pathway, that plays vital roles in many
physiological and pathological processes, such as cell differentiation, embryonic vascular development, tissue itself-renewal, the
occurrence of tumor and inflammation, and regulation of vascular function. Endothelial-to-mesenchymal transition (EndMT) is a
mechanism in which endothelial cells (ECs) lose their unique properties and acquire mesenchymal and stem cell-like characteristics under the influence of multiple factors. EndMT can be involved in a variety of pathologies, including embryonic development, malignant tumor invasion, and fibrotic disease. Recent findings have shown that inhibition of the NOTCH signaling pathway might accelerate the regression of IH and interfere with disease progression caused by EndMT. In this paper, the progression of endothelial-to-mesenchymal transition and NOTCH signaling pathway in IH were reviewed
Related Articles | Metrics
 Advances on autophagy mechanism in cell survival and regeneration after autologous fat grafting
JIA Xinyu, LI Facheng
2023, 19 (4):  420. 
Abstract ( 6 )   PDF (821KB) ( 7 )  
Autologous fat grafting has been widely used in plastic surgery. However, the unstable volume retention rate has
always been a significant problem, which is fundamentally due to the inadequate understanding of the mechanism of cell survival
and regeneration after transplantation. Autophagy plays a vital role in metabolic adaptation, cell differentiation and apoptosis inhibition, and many advances have been made in autophagy after autologous fat grafting. This review focused on the autophagy ofadipocytes and adipose-derived stem cells and the relationship between autophagy and inflammation and vascular remodeling. It
turned out that autophagy was closely related to cell survival and regeneration after autologous fat grafting, and moderately promoting autophagy may postoperatively improve the volume retention rate.
Related Articles | Metrics
 Clinical application of TAPAS flap in face reconstruction
YANG Jifan, FAN Jincai
2023, 19 (4):  425. 
Abstract ( 7 )   PDF (810KB) ( 5 )  
　Temporal artery postauricular skin flap, also known as TAPSA flap, is a technique for transposition of posterior auricular island flap, by pedicled with superficial temporal artery. Island TAPAS flap has been used in clinic practice since 1980s. After continuous innovation and combined with new technologies such as prefabricated flap, it has developed into a flap that could carry a variety of tissues at the same time for complex reconstruction. It has been used in the repair of organs such as nose, eyelid and periorbital reconstruction, which represents indispensable medical and aesthetic significance. In this paper, the clinical application of TAPAS flap in face reconstruction was reviewed.
Related Articles | Metrics
Research progress on rehabilitation effect of respiratory training on breast cancer-related lymphedema
LI Hui, YIN Yu, LI Chunxiao, et al
2023, 19 (4):  430. 
Abstract ( 12 )   PDF (839KB) ( 11 )  
　Breast cancer-related lymphedema (BCRL) is one of the common postoperative complications of breast cancer.
At present, there is no effective method to cure BCRL at home and abroad. Respiratory training, as a simple and easy training
program, can improve the lymphedema of patients through a variety of mechanisms, improve the quality of life, and may provide a
potential rehabilitation treatment for BCRL.
Related Articles | Metrics