Boling class in Nankai University is an experimental class with “the Plan for Cultivating Top-notch Students of Basic Disciplines”. Based on the importance and advantages of Boling class majored in Biological Sciences, the teaching group has carried out reform, such as adding the experiment teaching content, improving the experiment teaching mode and establishing the incentive mechanism for teachers and students to explore a new path of biochemical experiment teaching. These methods have effectively improved students’ innovative practical ability and teachers’ comprehensive business level. Simultaneously, a diversified laboratory management model was established to closely follow the pace of the reform in the experimental teaching. Reformed teaching system has been applied in biochemistry experiment to Boling class for four years, and good experiment teaching effect has been obtained. The reform not only improves students’ scientific literacy, but also stimulates students’ innovation ability, and has played an important role in the talent cultivation of Boling class.