To explore the novel teaching pattern of the experimental course, a composite of online course with flipped classroom and on-site experiment is proposed, and was applied to physiological science experiments course in Zhejiang University. The effects of novel teaching pattern was evaluated by a 7 scale questionnaire survey conducted among 359 students from 12 classes. Statistical results showed that the students rated highly the course design and agreed that the novel teaching pattern improved students’ interest in learning, promoted independent learning and active learning. The study demonstrated that students’ self-learning ability, in particular, practical ability, scientific writing ability have improved significantly under the novel teaching mode. Students’ knowledge comprehensive ability, capability for experimental design, questioning and problem-solving skills in scientific research were improved as well. The teaching pattern of physiological science experiments composed of online courses, flipped classroom and on-site experiments improves teaching quality and students’ comprehensive ability.