诊断学理论与实践

诊断学理论与实践 ›› 2017, Vol. 16 ›› Issue (03): 306-312.doi: 10.16150/j.1671-2870.2017.03.015

• 论著 • 上一篇    下一篇

微阵列酶联免疫荧光法和免疫印迹法检测过敏原特异性IgE的结果分析

赵婕, 赵凌旭, 胡晓波   

  1. 上海中医药大学附属龙华医院检验科,上海 200032
  • 收稿日期:2017-03-20 出版日期:2017-06-25 发布日期:2017-06-25
  • 通讯作者: 胡晓波 E-mail: huxiaobo@vip.sina.com

Analysis of performance of microfluid chip ELFA and IBT for detecting allergen specific IgE

ZHAO Jie, ZHAO lingxu, HU Xiaobo   

  1. Department of Clinical Laboratory, Longhua Hospital, Shanghai 200032, China
  • Received:2017-03-20 Online:2017-06-25 Published:2017-06-25

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摘要： 目的: 比较微阵列酶联免疫荧光法(enzyme-linked fluoroimmunoassay,ELFA)与免疫印迹法(immunoblotting test,IBT)2种过敏原筛查方法。方法: 利用多种IgE抗体强阳性混合人类血清和马血血清,对微阵列ELFA(以下简称ELFA)与IBT 这2种过敏原筛查方法的稳定性、稀释能力进行比对,并取其共有的过敏原进行阳性分级一致性比对。结果: ELFA检测尘螨、蟹、虾、花生、鸡蛋白、狗毛屑等6项过敏原的变异系数(coefficient of variation, CV%)均值明显低于IBT(T=6.20,P<0.01),而对于猫毛屑、蟑螂、牛奶等3项过敏原,ELFA的CV%均值略高于IBT,但差异尚无统计学意义(T=2.43,P>0.05)。对于不同稀释度的样本,2种方法的检测结果均与其稀释浓度呈正相关。2种方法间对屋尘螨及蟑螂的阳性分级一致性程度较高,而对花生、鸡蛋白、狗毛屑、猫毛屑、牛奶等5项过敏原的阳性分级一致性较差。结论: ELFA与IBT在过敏原检测中存在一定差异,但2种方法的检测结果均可很好地显示过敏患者血清中的特应性IgE浓度。与IBT相比,ELFA具有高稳定性、检测项目多、检测时间短、标本用量少等特点。

关键词: 特异性IgE, 酶联免疫荧光法, 免疫印迹法

Abstract: Objective: To compare the performance of BioIC Allergen specific IgE Detection Kit (micro-fluid chip enzyme-linked fluoroimmunoassay, ELFA) and EUROLINE Atopy China (immunoblotting test, IBT) for detecting allergen-specific IgE. Methods: The stability and dilution of these two methods were compared by using mixed human plasma with strong positivity for multi-sIgE and horse plasma, and the positive levels of common allergens were also compared. Results: The CV% of ELFA was obviously lower than that of IBT in six kinds of allergens including mite mix, crab, shrimp, peanut, egg white and dog dander(T=6.20, P<0.01), while was slight higher in cat dander,cockroach and milk(T=2.43, P>0.05). Both IBT and ELFA showed a positive correlation of testing result with concentration dilution. There was a high consistency of positive grading for mite mix and cockroach, while a weak consistency for peanuts and other five kinds of allergens. Conclusions: There are some differences between ELFA and IBT, but both could reflect the concentration of sIgE. Compared with IBT, ELFA has a higher stability and sensitivity, covering more allergens, less testing time and sample volume. ELFA could be used in allergen-specific IgE testing in clinical laboratory.

Key words: Allergen-specific IgE, Enzyme-linked fluoroimmunoassay, Immunoblotting test

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