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复发性自然流产患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序的表达

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  • 1.上海集爱遗传与不育诊疗中心,上海 200011;
    2.复旦大学附属妇产科医院检验科,上海 200011

收稿日期: 2017-04-12

  网络出版日期: 2017-06-25

Expression of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain on T cells in patients with recurrent spontaneous abortion

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  • 1. Shanghai Jiai Genetics & IVF Center, Shanghai 200011, China;
    2. Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University,Shanghai 200011, China

Received date: 2017-04-12

  Online published: 2017-06-25

摘要

目的: 探讨复发性自然流产(recurrent spontaneous abortion,RSA)患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序(T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain,TIGIT)的表达情况,为其可能作为治疗靶点提供理论依据。方法: 采集21例正常早孕者及23例RSA患者的抗凝外周血,分别检测其CD3+ T细胞表面分子TIGIT的百分比及细胞因子IL-10和IFN-γ的表达情况。结果: RSA组患者TIGIT+细胞所占CD3+ T细胞的百分比为(41.52±7.17)%,高于早孕组中的该百分比[(36.09±4.03)%],差异有统计学意义(P<0.05);RSA组TIGIT+ CD3+ T细胞中IL-10阳性细胞所占百分比分别为(11.68±5.90)%,低于早孕组TIGIT+ CD3+ T细胞中IL-10阳性细胞所占百分比[(16.33±6.65)%],差异有统计学意义(P<0.05);而CD3+ T细胞中IFN-γ的表达在2组间差异无统计学意义。结论: 负性共刺激分子TIGIT在RSA患者中异常表达,可能参与了母胎免疫的调控,与RSA的发生相关。

本文引用格式

陈颖, 李翠, 应春妹 . 复发性自然流产患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序的表达[J]. 诊断学理论与实践, 2017 , 16(03) : 273 -276 . DOI: 10.16150/j.1671-2870.2017.03.008

Abstract

Objective: To investigate the expression of TIGIT (T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain) on peripheral T cells in patients with recurrent spontaneous abortion(RSA). Methods: Proportion of TIGIT+ T cells and the expression of IFN-γ and IL-10 of TIGIT+ T cells were detected in 21 normal pregnant women and 23 RSA patients. Results: Compared with normal pregnant women, the proportion of TIGIT+ T cells were detected in RSA patients was significantly higher [(41.52±7.17)% vs (36.09±4.03)%)] (P<0.05), and the IL-10 expression of TIGIT+ T cells in RSA group were lower than normal pregnant group [(11.68±5.90)% vs (16.33±6.65)% ](P<0.05). The expression of IFN-γ in the two groups had no significant difference. Conclusions: The abnormal expression of TIGIT may be involved in the regulation of maternal-fetal immune tolerance and is related with the occurrence of RSA.

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