复发性自然流产患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序的表达

doi:10.16150/j.1671-2870.2017.03.008

诊断学理论与实践 ›› 2017, Vol. 16 ›› Issue (03): 273-276.doi: 10.16150/j.1671-2870.2017.03.008

复发性自然流产患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序的表达

陈颖¹, 李翠², 应春妹²

1.上海集爱遗传与不育诊疗中心,上海 200011;
2.复旦大学附属妇产科医院检验科,上海 200011

收稿日期:2017-04-12 出版日期:2017-06-25 发布日期:2017-06-25
通讯作者: 陈颖 E-mail: enya8@hotmail.com

Expression of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain on T cells in patients with recurrent spontaneous abortion

CHEN Ying¹, LI Cui², YING Chunmei²

1. Shanghai Jiai Genetics & IVF Center, Shanghai 200011, China;
2. Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University,Shanghai 200011, China

Received:2017-04-12 Online:2017-06-25 Published:2017-06-25

摘要/Abstract

摘要： 目的: 探讨复发性自然流产(recurrent spontaneous abortion,RSA)患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序(T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain,TIGIT)的表达情况,为其可能作为治疗靶点提供理论依据。方法: 采集21例正常早孕者及23例RSA患者的抗凝外周血,分别检测其CD3⁺ T细胞表面分子TIGIT的百分比及细胞因子IL-10和IFN-γ的表达情况。结果: RSA组患者TIGIT⁺细胞所占CD3⁺ T细胞的百分比为(41.52±7.17)%,高于早孕组中的该百分比[(36.09±4.03)%],差异有统计学意义(P<0.05);RSA组TIGIT⁺ CD3⁺ T细胞中IL-10阳性细胞所占百分比分别为(11.68±5.90)%,低于早孕组TIGIT⁺ CD3⁺ T细胞中IL-10阳性细胞所占百分比[(16.33±6.65)%],差异有统计学意义(P<0.05);而CD3⁺ T细胞中IFN-γ的表达在2组间差异无统计学意义。结论: 负性共刺激分子TIGIT在RSA患者中异常表达,可能参与了母胎免疫的调控,与RSA的发生相关。

关键词: 复发性自然流产, T细胞免疫球蛋白和免疫受体酪氨酸抑制基序, 机制

Abstract: Objective: To investigate the expression of TIGIT (T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain) on peripheral T cells in patients with recurrent spontaneous abortion(RSA). Methods: Proportion of TIGIT⁺ T cells and the expression of IFN-γ and IL-10 of TIGIT⁺ T cells were detected in 21 normal pregnant women and 23 RSA patients. Results: Compared with normal pregnant women, the proportion of TIGIT⁺ T cells were detected in RSA patients was significantly higher [(41.52±7.17)% vs (36.09±4.03)%)] (P<0.05), and the IL-10 expression of TIGIT⁺ T cells in RSA group were lower than normal pregnant group [(11.68±5.90)% vs (16.33±6.65)% ](P<0.05). The expression of IFN-γ in the two groups had no significant difference. Conclusions: The abnormal expression of TIGIT may be involved in the regulation of maternal-fetal immune tolerance and is related with the occurrence of RSA.

Key words: Recurrent spontaneous abortion, T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain, Mechanism

中图分类号:

R541.7

陈颖, 李翠, 应春妹. 复发性自然流产患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序的表达[J]. 诊断学理论与实践, 2017, 16(03): 273-276.

CHEN Ying, LI Cui, YING Chunmei. Expression of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain on T cells in patients with recurrent spontaneous abortion[J]. Journal of Diagnostics Concepts & Practice, 2017, 16(03): 273-276.

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复发性自然流产患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序的表达

Expression of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain on T cells in patients with recurrent spontaneous abortion

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