目的：研究叉头蛋白F1（forkhead box-F1,FOXF1）相邻非编码发育调控RNA（FOXF1 adjacent non-coding developmental regulatory RNA, FENDRR）在结肠直肠癌组织中的表达情况及其与患者预后间的关系,并探讨FENDRR与E型钙黏蛋白（E-cadherin蛋白）、N型钙黏蛋白（N-cadherin蛋白）间的相关性。方法：收集90例结肠直肠癌组织及配对的癌旁组织,采用实时荧光定量PCR（real time fluorescence quantitative polymerase chain reaction, rtfq-PCR）检测结肠直肠癌组织中FENDRR的表达情况,并分析其与患者临床病理参数之间的关系;采用蛋白印迹法检测结肠直肠癌组织中E-cadherin、N-cadherin蛋白的表达水平,并分析其与FENDRR表达之间的相关性。结果：长链非编码RNA（long no-coding RNA, lncRNA）FENDRR在结肠直肠癌组织中的相对表达水平为（8.29±0.13）,低于对应的癌旁组织的（8.59±0.18）,差异有统计学意义（t=13.666,P<0.01）。在结肠直肠癌组织中,E-cadherin蛋白与N-cadherin蛋白的表达呈负相关（r=-0.584,P<0.01）;FENDRR相对表达水平与E-cadherin蛋白的表达呈正相关（r=0.778,P<0.01）,而与N-cadherin蛋白的表达呈负相关（r=-0.692,P<0.01）,FENDRR高表达患者的生存期较低表达患者明显延长（90.0个月比 49.5个月,P=0.041）。结论：FENDRR在结肠直肠癌发生、发展过程中可能起抑癌基因的作用,且在该过程中FENDRR与E-cadherin、N-cadherin蛋白表达间有一定联系,FENDRR可能通过上皮间质转化过程来影响结肠直肠癌的转移;FENDRR高表达的结直肠癌患者生存期较低表达者长。
Objective: To investigate the expression of FENDRR, a long non-coding RNA gene, in colorectal cancer tissue and to detect the correlation of FENDRR expression with prognosis of colorectal cancer, and to explore the correlation between FENDRR and E-cadherin as well as N-cadherin. Methods: A total of 90 pairs of colorectal cancer tissue and matched para-carcinoma tissue samples were collected.The expression of FENDRR was detected by real time fluorescencequantitative polymerase chain reaction(rtfq-PCR). Western blotting analysis was used to detect the expression of E-cadherin and N-cadherin protein in colorectal cancer,and their correlations with FENDRR were analyzed. Results: The expression of FENDRR was decreased in cancer tissue (8.29±0.13) in relative to their corresponding para-cancer tissue(8.59±0.18), and the difference was statistically significant (t=13.666, P<0.01). E-cadherin was negatively correlated with N-cadherinin colorectal cancer tissue r=-0.584, P<0.01）; FENDRR expression was positively correlated with E-cadherin(r=0.778, P<0.01) and negatively correlated with N-cadherin protein (r=-0.692, P<0.01). The overall survival time of colorectal cancer patients with high FENDRR expression was higher than that of patients with low FENDRR expression (90.0 months vs 49.5 months, P=0.041). Conclusions: FENDRR shows an inhibiting effect on development of colorectal cancer, and has certain relationship with E-cadherin and N-cadherin.FENDRR might exert its inhibitory effect on metastasis by influencing the epithelial-mesenchymal transition(EMT) process. Detection of FENDRR might be useful for predicting the prognosis of colorectal cancer.
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