诊断学理论与实践

诊断学理论与实践 ›› 2018, Vol. 17 ›› Issue (01): 87-91.doi: 10.16150/j.1671-2870.2018.01.016

• 论著 • 上一篇    下一篇

长链非编码RNA FENDRR在结肠直肠癌中的表达及意义

陈滢a, 何莉莉b, 蒋锋c   

  1. 南京医科大学附属淮安市第一人民医院临床a. 检验科,b. B超室,c. 口腔科,江苏 淮安 223300
  • 收稿日期:2017-03-01 发布日期:2018-02-25
  • 通讯作者: 蒋 锋 E-mail: Jiang1276feng@163.com

Expression of long non-coding RNA FENDRR in human colorectal cancer and its correlation with prognosis

CHEN Yinga, HE Lilib, JIANG Fengc   

  1. a. Department of Clinical Laboratory, b. Department of Ultrasound, c. Department of Stomatology, Huai'an first People 's Hospital, Nanjing Medical University, Jiangsu Huai'an 223300, China
  • Received:2017-03-01 Published:2018-02-25

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摘要： 目的：研究叉头蛋白F1（forkhead box-F1,FOXF1）相邻非编码发育调控RNA（FOXF1 adjacent non-coding developmental regulatory RNA, FENDRR）在结肠直肠癌组织中的表达情况及其与患者预后间的关系,并探讨FENDRR与E型钙黏蛋白（E-cadherin蛋白）、N型钙黏蛋白（N-cadherin蛋白）间的相关性。方法：收集90例结肠直肠癌组织及配对的癌旁组织,采用实时荧光定量PCR（real time fluorescence quantitative polymerase chain reaction, rtfq-PCR）检测结肠直肠癌组织中FENDRR的表达情况,并分析其与患者临床病理参数之间的关系;采用蛋白印迹法检测结肠直肠癌组织中E-cadherin、N-cadherin蛋白的表达水平,并分析其与FENDRR表达之间的相关性。结果：长链非编码RNA（long no-coding RNA, lncRNA）FENDRR在结肠直肠癌组织中的相对表达水平为（8.29±0.13）,低于对应的癌旁组织的（8.59±0.18）,差异有统计学意义（t=13.666,P<0.01）。在结肠直肠癌组织中,E-cadherin蛋白与N-cadherin蛋白的表达呈负相关（r=-0.584,P<0.01）;FENDRR相对表达水平与E-cadherin蛋白的表达呈正相关（r=0.778,P<0.01）,而与N-cadherin蛋白的表达呈负相关（r=-0.692,P<0.01）,FENDRR高表达患者的生存期较低表达患者明显延长（90.0个月比 49.5个月,P=0.041）。结论：FENDRR在结肠直肠癌发生、发展过程中可能起抑癌基因的作用,且在该过程中FENDRR与E-cadherin、N-cadherin蛋白表达间有一定联系,FENDRR可能通过上皮间质转化过程来影响结肠直肠癌的转移;FENDRR高表达的结直肠癌患者生存期较低表达者长。

关键词: 结肠直肠癌, 长链非编码RNA, FOXF1相邻非编码发育调控RNA, 预后

Abstract: Objective: To investigate the expression of FENDRR, a long non-coding RNA gene, in colorectal cancer tissue and to detect the correlation of FENDRR expression with prognosis of colorectal cancer, and to explore the correlation between FENDRR and E-cadherin as well as N-cadherin. Methods: A total of 90 pairs of colorectal cancer tissue and matched para-carcinoma tissue samples were collected.The expression of FENDRR was detected by real time fluorescencequantitative polymerase chain reaction(rtfq-PCR). Western blotting analysis was used to detect the expression of E-cadherin and N-cadherin protein in colorectal cancer,and their correlations with FENDRR were analyzed. Results: The expression of FENDRR was decreased in cancer tissue (8.29±0.13) in relative to their corresponding para-cancer tissue(8.59±0.18), and the difference was statistically significant (t=13.666, P<0.01). E-cadherin was negatively correlated with N-cadherinin colorectal cancer tissue r=-0.584, P<0.01）; FENDRR expression was positively correlated with E-cadherin(r=0.778, P<0.01) and negatively correlated with N-cadherin protein (r=-0.692, P<0.01). The overall survival time of colorectal cancer patients with high FENDRR expression was higher than that of patients with low FENDRR expression (90.0 months vs 49.5 months, P=0.041). Conclusions: FENDRR shows an inhibiting effect on development of colorectal cancer, and has certain relationship with E-cadherin and N-cadherin.FENDRR might exert its inhibitory effect on metastasis by influencing the epithelial-mesenchymal transition(EMT) process. Detection of FENDRR might be useful for predicting the prognosis of colorectal cancer.

Key words: Colorectal cancer, Long non-coding RNA, FOXF1 adjacent non-coding developmental regulatory RNA, Prognosis

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