Objective: To evaluate the impact of implementation of "report and interpretation of rare pathogenic microorganism isolated" on clinical practice and communication between microbiology laboratory and clinicians. Methods: From Feb 2015, the microbiology laboratory of our hospital added a "report and interpretation of rare pathogenic microorganism isolated" form for the identification report when rare microorganism was isolated from clinical samples,which was considered to be of potential clinical significance. All the "report and interpretation of rare pathogenic microorganism isolated" issued during Feb 2015 to Nov 2016 were analyzed and the information of clinical data of patients and clinician's feedback were collected. SPSS Statistics 19.0 software was used to analyze the data. Results: From Feb 2015 to Nov 2016, 75 "report and interpretation of rare pathogenic microorganism isolated" were sent to 24 clinical departments, which involved 45 kinds of rare microorganism. Susceptibility test could not be performed routinely in Shanghai area on 93.3%(42/45) of the rare pathogens isolated. The average time from sample reception to delivery of the "report and interpretation of rare pathogenic microorganism isolated" was (3.5±1.5) days. After receiving the" report and interpretation of rare pathogenic microorganism isolated", 56.0%(42/75) of clinicians took related actions, with a higher ratio in 2016(62.7%, 37/59) than that in 2015(31.3%, 5/16) (P=0.024). Fifty-nine out of 75 patients from whom rare pathogen was isolated were clinically diagnosed as infectious disease.Patients who accepted therapy according to the recommendation offered with "report and interpretation of rare pathogenic microorganism isolated" had a higher successful rate than those who didn't(P<0.01). Conclusions: The "report and Interpretation of rare pathogenic microorganism isolated" has a positive role in clinical practice, and the clinician's acceptance for the "report and interpretation of rare pathogenic microorganism isolated" is increasing gradually.