Objective: To investigate the effect of silencing information regulator factor 1 (Sirtuin1, Sirt1) on acute myocardial infarction in mice and its mechanism. Methods: Model of acute myocardial infarction in mice were constructed by ligating the left anterior descending coronary artery. The infarcted and peripheral tissue were collected at different time after myocardial infarction (1, 3, 5 and 7 days) (the myocardial tissue of the sham operation group was taken as the control), and the expression of Sirt1 was detected by RT-PCR. In addition, in another series of model of myocardial infarction in mice, when successful ligation of coronary artery was established the Sirt1 overexpression lentivirus was injected into the tissue surrounding the infarction by microinjection (negative group of lentiviral injection served as the control). Myocardial infarction area was detected by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining seven days after operation. Mouse primary myocardial cells were cultured in vitro under hypoxia (2%O2), and resveratrol (Res) of different concentrations (25, 50 and 100 μmol/L) were used to stimulate the primary myocardial cells. The expression levels of Sirt1 and cysteine protease-3 shear bodies (cleaved caspase3) were detected by Western blotting, and cell apoptosis was detected by TUNEL method. Results: mRNA levels of Sirt1 in myocardial infarction and peripheral tissues were significantly decreased 1-7 days after infarction (P<0.05). Meanwhile, compared with the negative control group, the Sirt1 overexpression lentiviral injection group significantly decreased the area of infarction [(15.68±3.28)% vs (29.50±5.56)%, P<0.05)]. As the concentration of resveratrol increased, Sirt1 protein expression increased in a dose-dependent manner, while cleaved caspase-3 protein expression was decreased. The apoptosis of myocardial cells decreased gradually with the increase of concentration of resveratrol stimulation. Conclusions: The expression of Sirt1 declines in acute myocardial infarction. Overexpression of Sirt1 reduces the myocardial infarction area after acute myocardial infarction.
CHEN Yafen, CHEN Yuanyuan, WU Liping, XUE Qiqi, YANG Ke, LU Lin, CAO Jiumei
. Effect of silencing information regulator factor 1 on mice with acute myocardial infarction[J]. Journal of Diagnostics Concepts & Practice, 2018
, 17(06)
: 670
-675
.
DOI: 10.16150/j.1671-2870.2018.06.009
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