Objective To investigate general practitioner (GP) current understanding level of obstructive sleep apnea (OSA) and related influencing factors and evaluate the effect of online training on improving their knowledge and confidence levels of identifying and treatment of OSA. Methods Questionnaire surveys were used to evaluate GPs’ knowledge and understanding level of OSA before and after the training, and to analyze potential influencing factors. Results Before the training, the average score of questionnaires of 120 GP was (59.7±18.8) points, and the passing rate was 70.8%. After the training, the average score of questionnaires was increased to (75.0±9.6) points, and the passing rate reached 97.5%. The training significantly improved the GPs’ overall knowledge of OSA (P<0.05). The survey revealed that the training significantly enhanced GPs’ ability to identify high-risk OSA patients (P<0.05). Regarding future training needs, GP mainly expressed a desire to strengthen training in the use of OSA scoring scales, knowledge of non-invasive positive airway pressure (PAP) ventilation therapy, and the management of chronic diseases associated with OSA. Conclusions Online training significantly improved GPs’ knowledge and understanding level of OSA, enhanced their confidence in diagnosis and treatment of OSA. GP should actively participate in continuing education and training related to OSA.