Objective To analyze the therapeutic effect of endoscopic injection of tissue adhesive combined with hardener for the portal hypertensive patients with gastric varicose and prognosis. Methods A total of 84 patients with upper gastrointestinal hemorrhage and gastric varices diagnosed by gastroscopy during 2006 to 2015, who were performed endoscopic injection with tissue adhesive with hardener, were included in this study. The rate of gastric hemorrhage was compared before and after endoscopic treatment. The rate of gastric varices elimination and the prognosis were analyzed. Results There were 111 times of endoscopic treatment for 84 cases with an average of(1.32±0.68) times per case. Bleeding was (0.72±0.54) times/month before treatment and (0.30±0.82) times/month after treatment respectively with significant difference statistically(P<0.01). The eradication rate of varicose veins with almost disappearance was 75.0% during the period of 6 months post endoscopic treatment and 70.8% after 6 months of endoscopic treatment. No re-bleeding was found without any complications, ectopic embolization and infection after follow-up of (14.82±19.30) months. Conclusions Endosco-pic injection of tissue adhesive and hardener for gastric varices would reduce the rate of bleeding of gastric varicose and the varicose vein, which was an important procedure for prevention of re-bleeding of gastric varicose vein and could be applied clinically.