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| Cloning and Expression Analysis of smDREB3 Related to Stress Tolerance in Eggplant(Solanum melongena L.) |
| Xin-yu LIU, Hai-yan GE, Yang LIU, Hong-qiang HAN, Huo-ying CHEN |
| School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China |
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Abstract DREB has an important role in stress signal transduction pathways in plants.A cDNA fragment, named SmDREB3, was isolated from ‘YZ-14’ eggplant species using homology-based cloning method.The full length of cDNA of SmDREB is 887 bp, and the ORF is 777 bp, encoding 259 amino acids.It had 90% homologies with the sequence of potato and 88% homologies with the sequence of tomato. The mature protein isoelectric point was at pH 6.39 and molecular weight was 28.665 kDa.RT-qPCR analysis indicated that SmDREB3 was expressed in all organs.However, the expression level of SmDREB3 was tissue-specific.It expressed highest in the pericarp, followed by the leaf and sarcocarp, while it expressed relatively low in roots, stems and flowers.This study provides the theoretical basis for further researches on resilience mechanisms and cultivating new varieties that resist adverse environment.
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Received: 07 January 2015
Published: 08 March 2016
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2016, Vol. 34 
